PC12 cell integrated biosensing neuron devices for evaluating neuronal exocytosis function upon silver nanoparticles exposure  

作　　者：Pratikkumar Shah Qiaoli Yue Xuena Zhu Fangcheng Xu Huai-Sheng Wang Chen-Zhong Li 

机构地区：[1]College of Chemistry and Chemical Engineering, Liaocheng University [2]Nanobioengineering/Bioelectronics Laboratory [3]Department of Biomedical Engineering, Florida International University [4]Department of Chemical and Biochemical Engineering, College of Chemical Engineering, Xiamen University

出　　处：《Science China Chemistry》2015年第10期1600-1604,共5页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21273181,21328501);the Tai-Shan Scholar Research Fund of Shandong Province;the China Ocean Mineral Resources R&D Association project(DY125-15-T-08);the China Scholarship Council for their support

摘　　要：In this research, we explored a rapid assessment of silver nanoparticles(Ag-NPs) neurotoxicity at a single-cell level. Traditional nanotoxicity assays on large cell-populations may hide the important heterogeneity of individual cells often found in neuronal cells. The development in the area of new nanomaterial discoveries is far ahead of the development of advanced tools to measure these materials' toxicity. Development of alternative approaches to assess nanomaterials toxicity rapidly, reliably, and accurately is desirable. Here, we present a chip-based, cell-integrated microwell-array device for rapid assessment of neurotoxicity of Ag-NPs by monitoring the exocytosis function of a PC12 cell. Results presented here confirm the dose-dependent toxicity of Ag-NPs and the immediate alteration of their exocytosis function when exposed to NPs.In this research, we explored a rapid assessment of silver nanoparticles（Ag-NPs） neurotoxicity at a single-cell level. Traditional nanotoxicity assays on large cell-populations may hide the important heterogeneity of individual cells often found in neuronal cells. The development in the area of new nanomaterial discoveries is far ahead of the development of advanced tools to measure these materials＇ toxicity. Development of alternative approaches to assess nanomaterials toxicity rapidly, reliably, and accurately is desirable. Here, we present a chip-based, cell-integrated microwell-array device for rapid assessment of neurotoxicity of Ag-NPs by monitoring the exocytosis function of a PC12 cell. Results presented here confirm the dose-dependent toxicity of Ag-NPs and the immediate alteration of their exocytosis function when exposed to NPs.

关 键 词：neuron devices NANOTOXICITY AMPEROMETRY EXOCYTOSIS PC12 silver nanoparticle 

分 类 号：Q42[生物学—神经生物学] O614.122[生物学—生理学] TB383.1[理学—无机化学]