不同的甲醛杀菌浓度对A群、C群脑膜炎球菌荚膜多糖内毒素含量的影响  

The effect of different bactericidal concentration of formaldehyde solution on endotoxin content in group A and C meningococcal polysaccharides

作　　者：冯鹏[1] 冯佳丽[1] 王媛[1] 崔延智 魏振洲[1] 张宏[1] 陈作江[1] 赵海平[1] 

机构地区：[1]兰州生物制品研究所有限责任公司甘肃省疫苗工程技术研究中心,甘肃兰州730046

出　　处：《微生物学免疫学进展》2015年第6期14-17,共4页Progress In Microbiology and Immunology

摘　　要：目的研究不同的甲醛杀菌浓度对A群、C群脑膜炎球菌荚膜多糖内毒素含量的影响。方法将A群脑膜炎球菌发酵液分成A、B两组,A组采用体积分数为2.5%甲醛杀菌,B组采用体积分数为2.0%甲醛杀菌。将C群脑膜炎球菌发酵液分成C、D两组,C组采用体积分数为2.5%甲醛杀菌,D组采用体积分数为2.0%甲醛杀菌。分别纯化获得荚膜多糖,用动态浊度法测定荚膜多糖中内毒素的含量。结果 A、C两组荚膜多糖中内毒素含量显著低于B、D两组荚膜多糖中的内毒素含量(P<0.05)。结论使用不同浓度的甲醛杀菌,对A群、C群脑膜炎球菌荚膜多糖内毒素的含量有显著影响。较高浓度的甲醛利于菌体细胞的固定,从而防止细菌自溶释放内毒素,因而高浓度的甲醛能够减少其内毒素的含量,提高了产品质量。Objective To observe the effect of different bactericidal concentrations of formaldehyde solution on endotoxin content in group A and C meningococcal polysaccharides.Methods The fermentation broth for group A meningococcal were divided into two groups （ group A and B） , group A and B were sterilized by 2.5%formaldehyde solution and 2.0%formaldehyde solution, respectively.The fermentation broth for group C meningococcal were divided into group C and D, which were sterilized by 2.5%formaldehyde solution and 2.0%formaldehyde solution, respectively.Then, capsular poly-saccharides were purified respectively.The bacterial endotoxin content could be determined by a turbidimetric-kinetic meth-od.Results The endotoxin content in group A and group C meningococcals was significantly lower than in group B and D meningococcals （P〈0.05）.Conclusion Different bactericidal concentrations of formaldehyde solution have a significant effect on endotoxin content in group A and C meningococcal polysaccharides.High concentration of formaldehyde solution is helpful for fixing bacterial cells and then preventing the release of endotoxin from bacterial cell lysis.Thus high concentra-tion of formaldehyde solution could reduce endotoxin content in preparation and improve the quality of product.

关 键 词：A群 C群脑膜炎球菌荚膜多糖 内毒素 甲醛 动态浊度法 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ460.1