成骨细胞在激光立体成形多孔钛表面的附着、增殖及ALP活性  

Adhesion,proliferation and ALP activity of osteoblasts on porous Ti processed by laser solid forming

作　　者：刘智勇[1] 穆亚星 杨海欧[1] 高勃[2] 林鑫[1] 

机构地区：[1]西北工业大学凝固技术国家重点实验室,西安710072 [2]第四军医大学口腔医学院.军事口腔医学国家重点实验室

出　　处：《实用口腔医学杂志》2015年第5期603-606,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:51005181);国家重点基础研究发展计划(编号:2011CB610402)

摘　　要：目的:评价激光立体成形(LSF)的多孔钛对成骨细胞的生物相容性。方法:将SD大鼠成骨细胞接种于材料表面进行培养,对不同培养时间点成骨细胞在材料表面的黏附、增殖及ALP活性进行检测,并观察细胞形态。以经喷砂粗化处理的致密纯钛作为对照,用SPSS对试验结果进行统计分析。结果:培养2、3 h时实验组细胞贴附率高于对照组(P<0.05),4、7 d时细胞增殖和ALP活性明显高于对照组(P<0.05)。结论:激光成形的多孔钛材料具有良好的骨组织生物相容性。Objective：To study the biocompatibility of porous Ti processed by laser solid forming（LSF）with osteoblasts.Methods：SD rat osteoblasts were seeded on porous Ti surface processed by LSF.Adhesion,proliferation and ALP activity of the osteoblasts were measured at different time,and all samples were observed under SEM.Dense sand-blasted Ti sheets were used as the controls.All data were statistically analyzed.Results：Adhered cells on porous Ti were more than those on nonporous Ti sheets after 2 and 3 hours of culture（P 〈0.05）.At 4 and 7 days,cell proliferation and ALP activity were significantly higher on porous Ti than those on the controls（P 〈0.05）.Conclusion：Porous Ti processed by LSF possesses good biocompatibility with osteoblasts.

关 键 词：多孔钛 激光立体成形(LSF) 成骨细胞 生物相容性 

分 类 号：R783.1[医药卫生—口腔医学]