甲基丙烯酸β-羟乙酯对人牙髓细胞增殖和迁移的影响  

The effects of 2-hydroxyethyl methacrylate on the proliferation and migration of human dental pulp cells

作　　者：孙素[1] 方明[2] 沈丽娟[2] 王冠林[3] 第五海浪 肖玉鸿[1] 

机构地区：[1]成都军区昆明总医院口腔科.昆明医科大学教学医院,650032 [2]第四军医大学口腔医学院口腔修复科.军事口腔医学国家重点实验室 [3]昆明理工大学生命科学与技术学院

出　　处：《实用口腔医学杂志》2015年第5期648-652,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81460107;81130078)

摘　　要：目的：研究树脂单体甲基丙烯酸β-羟乙酯（HEMA）对人牙髓细胞（hDPCs）增殖和迁移的影响。方法：组织块法体外原代培养获得 hDPCs，以含不同浓度 HEMA 的 DMEM培养基与第3～5代 hDPCs 共同培养，用 MTT 实验检测 HEMA 作用于hDPCs 24、48、72 h 的增殖情况，Transwell 法观察细胞的迁移能力。结果：HEMA 在体外一定程度上抑制了 hDPCs 的增殖（与对照组比较，P ＜0．05），并呈时间浓度依赖性，24 h 组的细胞活力值均显著高于48 h 和72 h 组（P ＜0．05）。HEMA 减少了 hD-PCs 的迁移数（P ＜0．05）。结论：HEMA 体外能抑制人牙髓细胞的增殖和迁移。Objective：To assess the effects of HEMA on the proliferation and migration of human dental pulp cells（hDPCs）.Meth-ods：hDPCs were obtained using tissue explant culture technique in vitro,and then cells of the 3rd -5th passages were treated by differ-ent concentrations of HEMA for 24,48 and 72 h respectively.Cell proliferation was examined by MTT assay.Cell migration was ob-served by Transwell method.Results：The proliferation ability of hDPCs decreased when exposed to HEMA in both time and concentra-tion dependent manner（vs control,P 〈0.05）.Cell proliferation at 24 h expossure was statistically higher than that at 48 h and 72 h（P〈0.05）.The migration of hDPCs was significantly reduced in HEMA groups at different concentrations（vs control,P 〈0.05）.Con-clusion：HEMA inhibits the proliferation and migration of human dental pulp cells in vitro.

关 键 词：HEMA 牙髓细胞 增殖 迁移 

分 类 号：R783.1[医药卫生—口腔医学]