HBV相关肝癌组织HBsAg和HNF4α表达及其与组织学分化的关系  

Expression of HBsAg and hepatocyte nuclear factor 4 alpha in HBV-associated hepatocellular carcinomatissues

作　　者：朱云萌 冯骥良[1] 宋晨朝[1] 张世杰[1] 孙琳[1] 孟令佳[1] 刘巨超[1] 薛冉[1] 赵苗苗[1] 吕福东[1] 

机构地区：[1]首都医科大学附属北京佑安医院病理科,北京市100069

出　　处：《实用肝脏病杂志》2015年第5期517-520,共4页Journal of Practical Hepatology

基　　金：中国初级卫生保健基金会佑安肝病基金/艾滋病基金资助项目(编号:No.BJYAH-2011-071;No.BJYAH-2011-072)

摘　　要：目的：探讨在乙型肝炎病毒（HBV）相关肝细胞癌（HCC）患者癌组织中HBsAg和肝细胞核因子4α（HNF4α）表达及其与组织学分化的关系。方法回顾性分析2008年11月～2013年3月首都医科大学附属北京佑安医院经术后病理学检查证实为HCC组织256例。采用免疫组化法检测HBsAg、HNF4α和磷脂酰肌醇蛋白聚糖-3（GPC-3）表达。结果在256例HCC癌组织中，发现HBsAg阳性12例（4.7%）；在39例高分化、119例中分化和98例低分化肿瘤组织中，HBsAg阳性率分别为20.5%、1.7%和2.0%，前组显著高于后两组（P〈0.05）；HNF4α阳性百分比在高分化癌患者中为65.6%（21/32），显著高于中、低分化癌患者18.8%（3/16，P〈0.001）；在12例HBsAg阳性癌组织中，HNF4α阳性11例（91.7%），而在选择的36例HBsAg阴性癌组织中，HNF4α阳性13例（36.1%， P〈0.05）；在4例HBsAg阳性中分化和低分化HCC组织中，免疫组化显示GPC-3呈补丁样分布。结论 HBsAg在HBV相关HCC患者肿瘤细胞中的低表达可能与肿瘤细胞低表达HNF4α有关。Objective To investigate the expression of HBsAg and hepatocyte nuclear factor 4 alpha （HNF4α） in HBV-associated hepatocellular carcinoma （HCC） tissues. Methods The liver cancerous tissues of 256 individuals with HBV-associated HCC from 2008 to 2013 in You-An Hospital affiliated to Capital Medical University were retrospectively reviewed. The expression of HBsAg,HNF4α and glypican-3（GPC-3） were immuno-histocamically stained. Results 12 （4.7%）out of 256 were found HBsAg positive in tumor tissues; the HBsAg-positive rates in 39 well-differentiated,119 moderately and 98 poorly （2%,2/98） differentiated cancerous tissues were 20.5%（P〈0.05）,1.7% and 2.0%,respectively;the positive rates of HNF4α in well-differentiated tumor was 65.6%（21/32）,much higher than in moderately or poorly differentiated cancerous tissues [18.8%（3/16）,P〈0.001];11 （91.7%）out of 12 HBsAg positive caner were HNF4α positive,much higher than 36.1%（P〈0.05） in 36 with HBsAg negative;the expression of GPC-3 was positive in 4 moderately or poorly differentiated cancerous tissues. Conclusion The low expression of HNF4α might be related to the low expression of HBsAg in HBV-associated HCC tissues,which means the expression of HBsAg should be assisted by the existence of HNF4α.

关 键 词：肝细胞癌 肝细胞核因子4Α 

分 类 号：R735.7[医药卫生—肿瘤]