一株甲醛降解菌的分离鉴定及其降解特性分析  

Isolation and Identification of a Formaldehyde-degrading Bacteria and Analysis of Its Degrading Characteristics

作　　者：陈晓飞[1] 冯菲[1] 孙玉飞[1] 王雪妍[1] 周伏忠[1] 

机构地区：[1]河南省微生物工程重点实验室,郑州450008

出　　处：《河南科学》2015年第9期1533-1538,共6页Henan Science

基　　金：河南省社会发展项目(122102310505)

摘　　要：以甲醛为唯一碳源和能源,从皮革废水处理厂的活性污泥中,分离得到一株甲醛高效降解菌JQ-6,结合形态学特征、生理生化特性研究和16S r DNA序列鉴定显示,JQ-6属于恶臭假单胞菌(Pseudomonas putida).通过单因素试验和正交试验,检测不同培养条件对菌株降解甲醛的影响,得出菌株JQ-6降解甲醛的最佳条件为:甲醛1000 mg/L,30℃、p H6.0、摇床转速150 r/min、100 m L培养基JQ-6菌悬液的接种量为3 m L,在此条件下培养24 h后,菌株JQ-6对甲醛的降解率可以达到70%以上.A bacterial strain（JQ-6）capable to degrade and metabolize formaldehyde as a sole carbon and energy sources was isolated from the collected activated sludge of leather wastewater treatment plants. The results of morpho-logical features,physiological and biochemical characters as well as 16S rDNA sequence analysis of the bacterium showed that,the JQ-6 strain was Pseudomonas putida. Single-factor and orthogonal experiments were employed to study the capability of degradation characteristics of strain JQ-6. The optimum conditions of formaldehyde degradation were as follow：formaldehyde concentration of 1000 mg/L,incubation temperature of 30℃,pH 6.0,rotating rate of 150 r/min,100 mL fresh medium seeded with 3 mL JQ-6 bacterial suspension. The formaldehyde degradation rate reached 70%within 24 h by this strain.

关 键 词：甲醛降解 分离鉴定 降解特性 恶臭假单胞菌 

分 类 号：X172[环境科学与工程—环境科学]