CD4^+CD25^+Foxp3^+调节性T细胞在子宫内膜癌患者外周血中的表达及意义  

Level of CD4^+CD25^+Foxp3^+ Treg cells in peripheral blood and their role to the patient with endometrial carcinoma

作　　者：张雅丽[1] 侯安丽[1] 王杏茶[1] 许倩[2] 岳志玲 王会民[3] 潭小波 

机构地区：[1]承德医学院附属医院妇科,承德067000 [2]承德医学院基础医学研究所,承德067000 [3]承德医学院附属医院产科,承德067000 [4]承德医学院附属医院眼科,承德067000

出　　处：《现代免疫学》2015年第5期410-414,共5页Current Immunology

基　　金：河北省科技项目((20122136)

摘　　要：探讨在子宫内膜癌患者外周血中CD4+CD25+Foxp3+调节性T细胞的表达情况及意义。采用流式细胞术检测84例术前子宫内膜癌患者及40例子宫肌瘤患者外周血中CD4+CD25+Foxp3+细胞比例及Foxp3平均荧光强度,采用qRT-PCR检测两组患者外周血中Foxp3的mRNA表达情况,同时采用ELISA检测外周血中TGF-β1和IL-17含量。与子宫肌瘤组比较,子宫内膜癌患者外周血中CD4+CD25+Foxp3+Treg细胞的比例虽略有升高但没有统计学意义(P=0.08),而CD4+CD25+细胞内Foxp3的平均荧光强度明显升高(P<0.001)。子宫内膜癌患者外周血中Foxp3的mRNA表达要明显多于子宫肌瘤组(P<0.001)。子宫内膜癌患者外周血中TGF-β1、IL-17的含量要多于子宫肌瘤组。子宫内膜癌患者外周血中的Foxp3+Treg细胞表达增多,这些细胞可能通过增加细胞因子TGF-β和IL-17的分泌从而调节机体对肿瘤细胞免疫反应的方向,最终促进子宫内膜癌的发生和发展。To investigate the level of CD4^＋CD25^＋Foxp3^＋ Treg cells in peripheral blood and their role to the patient with endometrial carcinoma. The peripheral blood samples were collected from 84 patients with endometrial carcinoma and 40 patiens with hysteromyoma. The percentage of Treg cells and the mean fluorescence intensity （MFI） of Foxp3 were detected with flow cytometry, the level of Foxp3 mRNA was examined with qRT-PCR,and the secretion of TGF-β1 and IL-17 was evaluated with ELISA. The percentage of Treg cells in peripheral blood of patients with endometrial carcinoma was higher than that of uterine myoma,which had no statistical significance between the two groups. However, the MFI of Foxp3, the level of Foxp3 mRNA, and the production of TGF-β1 and IL-17 in peripheral blood of patients with endometrial carcinoma was significantly increased. The level of Treg ceils in peripheral blood of the patients with endometrial carcinoma was increased, which may enhance the secretion of their downstream cytokines of TGF-β and IL-17, hereby regulate the response of the body to carcinoma ceils and eventually promote the development of endometrial carcinoma.

关 键 词：CD4^+CD25^+Foxp3^+Treg细胞 子宫内膜癌 TGF-Β IL-17 

分 类 号：R737.33[医药卫生—肿瘤]