体外压力刺激对血管内皮细胞ET-1、PGI2、IFN-β和TNF-α合成释放影响的研究  

Study of Effects in External Pressure Stimulation on the Synthetize and Release of ET-1,PGI2,IFN-β and TNF-αof Vascular Endothelial Cells

作　　者：江瑜[1] 陈波[2] 李程[2] 陈磊[2] 胡铁汉 

机构地区：[1]贵阳中医学院第一附属医院,贵州贵阳550004 [2]贵阳中医学院针灸推拿学院,贵州贵阳550025

出　　处：《按摩与康复医学》2015年第18期134-137,共4页Chinese Manipulation and Rehabilitation Medicine

基　　金：贵州省科学技术基金,编号:黔科合J字【2011】2304号;贵州省高层次人才科研条件特助经费项目,编号:TZJF-2011年-29号

摘　　要：目的：探讨针灸推拿体外压刺激对血管内皮细胞ET-1、PGI2、IFN-β和TNF-α的影响.方法：体外培养血管内皮细胞,平分为空白对照组、低压力力学刺激组和高压力刺激组,分别予以0kPa、100kPa和200kPa压力刺激,检测细胞外培养液血管活性物质ET-1、PGI2、IFN-β和TNF-α含量.结果：低、高压力刺激组PGI2合成释放量均显著少于空白对照组（P＜0.01）,高压力刺激组ET-1合成释放多于空白对照组（P＜0.05）;高压力刺激组IFN-β合成释放量显著多于空白对照组（P＜0.05）,低、高压力刺激组TNF-α合成释放量均多于空白对照组（P＜0.05）.结论：压力刺激调节血管内皮细胞血管舒缩活性物质ET-1、PGI2合成释放,促进具有免疫调节功能的细胞因子IFN-β、TNF-α合成释放,可能是针灸推拿发挥＂运气血、扶助正气＂作用的细胞生物力学原理之一。Objective：To investigate the effects of acupuncture and massage on ET-1, PGI2, IFN-βand TNF-αof vascular endothelial cells. Methods：The vascular endothelial cells were cultured external and divided into blank control group, low pressure mechanical stimulation group and high pres-sure stimulation group, respectively with 0kPa, 100kPA, 200kPa pressure stimulation, content of vasoactive substance ET-1, PGI2, IFN-βand TNF-αof extracellular nutrient solution was detected. Results：The synthetize and release of PGI2 in low and high pressure stimulation group were both sig-nificantly less than that in blank control group （P＜0.01）, and ET-1of high pressure stimulation group was more than that of blank control group （P＜0.05）;IFN-βof high pressure stimulation group was significantly more than that of blank control group （P＜0.05）, TNF-αof low and high pressure stimulation group was more than that of blank control group （P＜0.05）. Conclusion：Pressure stimulation regulates the synthetize and release of vaso-motor active substances ET-1 and PGI2 of vascular endothelial cells, and promotes the synthetize and release of cytokines IFN-βand TNF-αwith im-mune regulating function, which could be one of biomechanical principles of acupuncture and massage playing a role of ＆quot; carrying qi and blood, strengthening genuine qi＆quot;.

关 键 词：血管内皮细胞 ET-1 PGI2 IFN-Β TNF-Α 针灸 推拿 力学刺激 

分 类 号：R-33[医药卫生]