激活素A与NGF协同刺激鸡胚背根神经节神经突起生长  

Role of activin A and NGF in co-stimulating neurite outgrowth of dorsal root ganglia ofembryonic chicken

作　　者：方琳[1] 王轶楠[2] 葛敬岩[2] 刘海岩[2] 李晨光[2] 李继茹[2] 柳忠辉[2] 

机构地区：[1]长春医学高等专科学校基础医学部,长春130031 [2]吉林大学基础医学院免疫系,长春130021

出　　处：《中国免疫学杂志》2015年第9期1206-1209,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.81273199;81371445);吉林省科技厅课题(No.20140520012JH);吉林省卫生厅课题(No.2013Q007)资助

摘　　要：目的:探讨激活素A(Activin A)与神经生长因子(Nerve growth factor,NGF)共同刺激鸡胚背根神经节(DRG)神经突起生长作用。方法:采用8 d的鸡胚DRG原代培养法,通过Activin A与NGF联合刺激,观察DRG神经突起生长和DRG神经元存活情况。采用RT-PCR检测钙基因相关肽(CGRP)。结果:Activin A与NGF体外联合培养3 d时DRG神经突起生长比单纯NGF组更明显,DRG神经元存活数量也明显增加,Activin A与NGF联合作用可以明显促进CGRP mRNA表达。结论:Activin A对NGF诱导的鸡胚DRG神经突起生长和维持DRG神经元存活具有增强作用,提示二者的联合应用可能为治疗神经元损伤及变性疾病的应用提供了新的数据和实验依据。Objective：To investigate the effect of activin A and nerve growth factor （ NGF） NGF on stimulating neurite outgrowth of dorsal root ganglia（DRG）of the embryonic chicken.Methods：In this study,we observed that activin A and NGF together induced neurite outgrowth of DRG and kept survival of DRG neurons by the primary cultured DRGs from embryonic day 8 （ E8 ） chicken.calcitonin gene-related peptide（CGRP）CGRP mRNA expressions were analyzed by RT-PCR.Results： The DRG treated with activin A ＋NGF had obvious neurite outgrowth ,compared with only NGF group on day 3,and the number of living DRG neurons also increased.Activin A ＋NGF up-regulated the mRNA expressions of CGRP in DRG.Conclusion：The Data demonstrated that activin A with NGF can synergistically stimulate DRG neurite outgrowth and maintain the DRG neurons survival , suggesting that it is more effective that NGF and activin A together treat the associated disease of nerve system.

关 键 词：激活素A 神经生长因子 神经突起 钙基因相关肽 

分 类 号：R742.1[医药卫生—神经病学与精神病学]