少突胶质谱系细胞的生物学特性  

The biological characteristics of oligodendrocyte lineage cells

作　　者：卢玉仙[1] 夏春林[2] 高薇[1] 徐益荣[1] 

机构地区：[1]盐城卫生职业技术学院,江苏盐城224005 [2]苏州大学医学部,江苏苏州215123

出　　处：《解剖科学进展》2015年第5期505-508,共4页Progress of Anatomical Sciences

摘　　要：目的研究少突胶质谱系细胞定向分化过程中的增殖及形态学的变化,为进一步研究脑室周围白质软化的临床治疗奠定基础。方法依据各种胶质细胞黏附性及生长时间的差异,通过机械振荡法和差速贴壁法从原代培养中获得纯化的O-2A祖细胞,接种于含有血小板源性生长因子和碱性成纤维细胞生长因子的培养液观察其增殖情况,再以三碘甲状腺原氨酸和睫状神经营养因子定向诱导分化,并观察分化过程中细胞的形态学变化,免疫细胞化学鉴定。结果纯化的O-2A祖细胞接种后指数式增殖呈团,细胞界限不清,表达NG2,至第4天细胞不再增多进入分裂终期;进入分化过程的少突胶质谱系细胞突起逐渐分级、交互,免疫化学鉴定依次表达O4、Gal C及MBP。结论 O-2A祖细胞具有良好的增殖能力,沿着少突胶质谱系细胞定向分化伴随形态学及表面表达抗原的变化。Objective To study the proliferation and morphological changes of oligodendrocyte lineage cells in the process of differentiation, to lay the groundwork for the study of the periventricular leukomalacia for clinical treatment. Methods Based on the differential properties of developmental time-course and cellular adhesions, O-2A progenitor cells were achieved by mechanical shaking and differential adhesion method. The platelet-derived growth factor and basic fibroblast growth factor were added into the culture medium, the differentiation of O-2A progenitor cells was induced by three iodothyronine and ciliary neurotrophic factor. The morphological change and biological characteristics of the cells were observed with immunocytochemical identification. Results The proliferation of purified O-2A progenitor cells was observed and expresses NG2 positively, with no proliferation at the fourth day. The O-2A progenitor cells were finally differentiated into oligodendrocyte lineage cells expressed specific antigens（O4、GalC and MBP） positively. Conclusion O-2A progenitor cells might have strong ability of proliferation and differentiate into oligodendrocyte accompanied by surface antigen expression.

关 键 词：少突胶质谱系细胞 O-2A祖细胞 增殖 分化 

分 类 号：R742.8[医药卫生—神经病学与精神病学]