HPLC法测定薜荔中儿茶素的含量  

Determination of Catechin in Ficus Pumila Linn

作　　者：夏爱军[1] 韦少宣[2] 廖厚知 

机构地区：[1]解放军303医院药剂科,广西南宁530021 [2]广西中医学院药学院中药教研室,广西南宁530021 [3]广西汇科药物研究所,广西南宁530007

出　　处：《解放军药学学报》2015年第4期333-334,337,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立薜荔中儿茶素的含量测定方法。方法采用HPLC法进行测定,色谱柱为Agilent C18（4.6 mm×250 mm,5μm）;流动相为乙腈-0.4%磷酸溶液（10∶90）;流速为1.0 ml·min-1;检测波长为280 nm。结果薜荔中儿茶素含量在10.05～90.45μg·ml-1范围内线性关系良好（r=0.9998）;平均回收率为99.17%,RSD为1.01%。结论所建立的方法简便快速、准确、灵敏、重复性好,可用于薜荔中儿茶素的含量测定。Objective To establish a method for determination of catechin content in Ficus Pumila Linn. Methods Agilent C18 （4.6 mm · 250 mm,5 μm） colmnn was used. The mobile phase was acetonitrile-0.4% phosphonic acid solution （10： 90） at a flow rate of 1.0 ml·min-1. The detection wavelength was 280 nm. Results The linearity of hyperin was good within the range of 10.05 -90.45 μg·m1-1. The average recovery was 99.17% , while RSD was 1.01%. Conclusion This method is accurate, sensitive and easily reproducible. It can be applied to quality control in Ficus Pumila Linn.

关 键 词：薜荔 儿茶素 HPLC 

分 类 号：R927.2[医药卫生—药学]