肝炎肝硬化骨髓祖细胞变化与血清ALT及LDH相关性研究  

Changes of hepatitis cirrhosis bone marrow progenitor cells and correlation between serum ALT and LDH

作　　者：景晔[1] 徐和福[1] 陆光生[1] 宋丽萍[1] 杨辉[1] 

机构地区：[1]北京军区天津疗养院,天津300381

出　　处：《生物医学工程与临床》2015年第5期508-510,共3页Biomedical Engineering and Clinical Medicine

摘　　要：目的 探讨肝炎肝硬化骨髓祖细胞变化对血清乳酸脱氢酶（LDH）的影响及其临床意义。方法 随机选择代偿期及失代偿期肝炎肝硬化各13例，共26例患者，其中男性21例，女性5例；年龄26 ～ 58岁，平均年龄46岁。对照组10例，其中男性8例，女性2例；年龄31 ～ 52岁，平均年龄39岁。进行骨髓晚期红系祖细胞（CFU-E）、粒-单系祖细胞（CFU-GM）及成纤维祖细胞（CFU-F）体外培养，血清丙氨酸转氨酶（ALT）及LDH检测。结果 代偿期及失代偿期肝炎肝硬化CFU-E、CFU-GM及CFU-F集落生长率两组比较[（33.21 ± 24.85） × 10^4、（49.38 ± 26.21） × 10^5、（30.98 ± 15.44） × 10^5 vs （9.74 ± 6.85） × 10^4、（31.42 ± 11.78） × 10^5、（21.06 ± 10.47） × 10^5]，差异有统计学意义（P 〈 0.05）。三系祖细胞减少组ALT水平明显高于正常组（P 〈 0.01）；而LDH水平比较，差异无统计学意义（P 〉 0.05）。Hb降低组LDH水平明显高于Hb正常组，差异有统计学意义（P 〈 0.05）；中性粒细胞降低组LDH水平明显高于正常组，差异有统计学意义（P 〈 0.05）结论 检测LDH活性对判断肝炎肝硬化骨髓祖细胞生长程度及监测其预后是一个很有价值的指标。Objective To investigate hepatitis cirrhosis bone marrow progenitor cells change on the influence of serum lactate dehydrogenase（LDH） and its clinical significance. Methods A total of 26 patients with compensated cirrhosis（n = 13） and decompensated cirrhosis（n = 13） were enrolled, which included 21 males and 5 females, aged 26- 58 years old with mean age of46 years old. Ten healthy cases were set as control group, which included 8 males and 2 females, aged 31- 52 years old with mean age of 39 years old. The colony-forming unit-erythroid（CFU-E）, colony- forming unit-granulocyte macrophage（CFU-GM）and colony-forming unit-fibroblast（CFU-F） were cultured in vitro. The serum alanine aminotransferase（ALT） and LDH were detected. Results Compared the colony growth rate of CFU-E, CFU-GM and CFU-F between compensated cirrhosis and decompensated cirrhosis[（33.21 ± 24.85） × 104,（49.38 ± 26.21） × 105,（30.98 ± 15.44） × 105vs（9.74 ± 6.85） × 104,（31.42 ± 11.78） ×105,（21.06 ± 10.47） × 105], the difference was statistically significant（P 0.05）. ALT level in the three progenitor cells groups decreased significantly which was higher than that in normal group（P 0.01）, and about LDH levels, there was no statistical significance（P 0.05）. The LDH levels in Hb decreased group was significant higher than that in Hb normal group, the difference was statistically significant（P 0.05）, and LDH levels in neutronpenia group was significant higher than that in normal group, the difference was statistically significant（P 0.05）. Conclusion It is demonstrated that detecting LDH activity of hepatitis cirrhosis bone marrow progenitor cell growth and monitoring its prognosis is the valuable indicator.

关 键 词：肝炎肝硬化 祖细胞 乳酸脱氢酶 

分 类 号：R575.2[医药卫生—消化系统] R446[医药卫生—内科学]