琥珀酸在大鼠海马CA1区对离子型Glu受体介导的兴奋性突触后电流的调节作用  

Regulation of succinic acid on ionotropic Glu receptor-mediated excitatory postsynaptic currents in rat hippocampal CA1 neurons

作　　者：谷士军 辛奎波 薛秀梅 孙玲玉[2] 李晓玲[3] 丛红群[2] 岳旺[2] 隋忠庆 

机构地区：[1]烟台市心理康复医院,山东烟台265200 [2]青岛大学医学院 [3]青岛市市立医院

出　　处：《中国民康医学》2015年第19期64-65,70,共3页Medical Journal of Chinese People’s Health

基　　金：烟台市科技局资助(项目编号:2012107);国家自然科学基金(No.30572178)资助项目

摘　　要：目的:研究琥珀酸对大鼠海马CA1区的离子型Glu受体介导的兴奋性突触后电流的调节。方法:采用红外可视脑片膜片钳技术,观察琥珀酸对大鼠海马CA1区的谷氨酸(Glu)能自发兴奋性突触后电流(s EPSCs)和微小兴奋性突触后电流(m EPSCs)的调节作用。结果:琥珀酸能降低大鼠海马CA1区离子型Glu受体介导的s EPSCs和m EPSCs的电流幅度,而对其频率和半衰减时间均无影响。1μmol/L琥珀酸组s EPSCs和m EPSCs的电流幅度分别为(24.16±2.61)p A和(23.36±2.73)p A,与对照组比较差异显著(P<0.01)。结论:琥珀酸对突触前Glu释放无影响,但可显著降低EPSCs的电流幅度,琥珀酸可作为一种神经调质影响突触后兴奋性活动。Objective:To study regulation of succinic acid on ionotropic Glu-mediated miniature excitatory postsynaptic cur-rents ( mEPSCs) and spontaneous excitatory postsynaptic currents ( sEPSCs) in rat hippocampal CA1 neurons. Methods:The regula-tion of succinic acid on ionotropic Glu-mediated mEPSCs and sEPSCs in the rat hippocampal CA1 neurons were observed by using in-frared visual patch clamp technique. Results:Succinic acid could reduce the amplitudes of ionotropic Glu-mediated mEPSCs and sEP-SCs, but had no effect on the frequency and half-decay period. The amplitudes of mEPSCs and sEPSCs were (23. 36±2. 73) pA and (24. 16±2. 61) pA in the 1 μmol/L Succinic acid group, which had significant differences with control group (n=12, P<0. 01). Conclusions:Succinic acid has no effect on presynaptic Glu release, but can significantly reduce the current amplitude. Therefore, it may be a neuromodulator influencing postsynaptic excitatory activities.

关 键 词：琥珀酸 海马 兴奋性突触后电流 谷氨酸 

分 类 号：R-332[医药卫生]