联合检测Ang-2和GP73对原发性肝癌诊断与鉴别的临床价值  

Diagnostic value of combined detection of angiopoietin-2 and Golgi protein 73 in primary liver cancer

作　　者：赛文莉[1] 陈雯[2] 姚登福[1] 邱历伟[1] 吴玮[1] 王理[1] 杨君伶[1] 张海健[1] 郑文杰[1] 

机构地区：[1]南通大学附属医院临床医学研究中心,江苏省南通市226001 [2]南通大学附属医院检验科,江苏省南通市226001

出　　处：《世界华人消化杂志》2015年第25期4032-4040,共9页World Chinese Journal of Digestology

基　　金：南通市科技局基金资助项目;No.BK2013048~~

摘　　要：目的:定量原发性肝癌(primary liver cancer,PLC)患者外周血中血管生成素-2(angiopoietin-2,Ang-2)和高尔基体蛋白73(Golgi protein 73,GP73)表达水平,探讨对PLC诊断与鉴别的临床价值.方法:以酶联免疫吸附法(ELISA)检测急、慢性肝炎,肝硬化及PLC患者外周血Ang-2和GP73浓度;以免疫组织化学分析肝癌及癌周组织Ang-2和GP73表达,并以Western blot证实.结果:肝癌组织中呈棕黄色或棕褐色颗粒的GP73和Ang-2阳性表达,主要分布于胞浆或胞膜上,并经Western blot证实癌组织均明显高于周围组织(P<0.001).PLC患者外周血Ang-2水平为40.8 ng/m L±3.5 ng/m L,GP73为87.3ng/m L±78.4 ng/m L,均显著高于正常对照、急性肝炎、慢性肝炎和肝硬化组(P<0.01);且Ang-2与GP73改变呈显著正相关(r=0.987,P=0.013);受试者工作特征曲线下面积Ang-2=0.915,GP73=0.899,与甲胎蛋白(α-fetoprotein,AFP)联检对PLC具有互补诊断价值.结论:Ang-2和GP73过表达与PLC进展密切相关,且有助于PLC的临床诊断与鉴别诊断.AIM： To detect the expression of circulating angiopoietin-2 （Ang-2） and Golgi protein 73 （GP73） in patients with primary liver cancer and to analyze their value in the diagnosis and differential diagnosis of primary liver cancer （PLC）. METHODS： The levels of circulating Ang-2 and GP73 expression were detected by ELISA in patients with PLC, acute hepatitis, chronic hepatitis, or liver cirrhosis. The expression of Ang-2 and GP73 in cancerous and surrounding noncancerous tissues was analyzed by immunohistochemistry and confirmed by Western blot. RESULTS： Positive expression of hepatic GP73 and Ang-2 was characterized by the presence of brown granules in the cytoplasm or cytomembrane. Hepatic GP73 and Ang-2 expression was significantly higher in cancerous tissue than in surrounding noncancerous tissue （P 〈 0.01）. The levels of circulating Ang-2 and GP73 in PLC patients （40.8 ng/mL ± 3.5 ng/mL and 87.3 ng/mL ±78.4 ng/mL, respectively） were significantly higher （P 〈 0.01） than those in normal subjects and patients with acute hepatitis, chronic hepatitis, or liver cirrhosis. There was a positive correlation between Ang-2 and GP73 （r = 0.987, P = 0.013）. The area under the receiver operating curve （ROC） for the diagnosis of PLC was 0.915 for Ang-2 and 0.899 for GP73. CONCLUSION： The overexpression of Ang-2 and GP73 is associated with HCC development and may be useful biomarkers for PLC diagnosis or differential diagnosis.

关 键 词：原发性肝癌 血管生成素-2 高尔基体蛋白73 酶联免疫吸附法 诊断 

分 类 号：R735.7[医药卫生—肿瘤]