一种高效省本的非变性聚丙烯酰胺凝胶电泳银染法的建立——以水稻为例  

A High-efficiency and Low Cost Method of DNA Silver Staining in Non-denaturant Polyacrylamide Gel Electrophoresis——An Example Based on Rice

作　　者：王运斌 江良荣[1] 黄荣裕[1] 黄育民[1] 郑景生[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361102

出　　处：《福建稻麦科技》2015年第3期4-8,共5页Fujian Science and Technology of Rice and Wheat

基　　金：福建省自然科学基金计划项目(2012J01153);福建省高校产学合作重大科技计划项目(2011N5013);福建省科技重大专项(2013NZ0002-2)

摘　　要：非变性聚丙烯酰胺凝胶电泳银染技术已广泛用于DNA片段检测。介绍一种本实验室改进的高效省本的非变性聚丙烯酰胺凝胶电泳银染方法,该法将固定和染色两步骤合并,整个银染过程只需16 min即可完成。同时,该法无需使用无水乙醇和冰乙酸,减少了硝酸银、氢氧化钠及甲醛的使用量。A method of silver staining in non-denaturant polyacrylamide gel electrophoresis has widely been used to detect DNA fragments. Here we developed a high-efficiency and low cost method of DNA silver staining in non-denaturant polyacrylamide gel electrophoresis. The whole new procedure of silver staining has combined two steps of fixation and staining into one step and can be completed within 16 min. Furthermore, the new procedure eliminates the use of ethanol and acetic acid and reduces the use amount of silver nitrate, sodium hydroxide and formaldehyde in silver steps.

关 键 词：非变性聚丙烯酰胺凝胶 电泳 银染 

分 类 号：Q94-33[生物学—植物学]