磷脂酰胆碱在伴放线放线杆菌粘附和侵袭过程中的作用  

Role of phosphorylcholine in Aggregatibacter actinomycetemcomitans adhesion and invasion

作　　者：王勤[1] 张雄[1] 钟德钰[1] 轩东英[1] 谢成婕[1] 刘伟珍[1] 章锦才[1] 

机构地区：[1]广东省口腔医院.南方医科大学附属口腔医院牙周科,广东广州510280

出　　处：《广东牙病防治》2015年第9期457-461,共5页Journal of Dental Prevention and Treatment

基　　金：国家自然科学基金项目(81371151);广东省医学科学技术研究基金项目(A2014112)

摘　　要：目的探讨磷脂酰胆碱在伴放线放线杆菌(Aggregatibacter actinomycetemcomitans,Aa)对人脐静脉血管内皮细胞粘附和侵袭过程中的作用。方法采集7例侵袭性牙周炎患者牙周袋内的非附着性菌斑,接种于Aa选择性培养板上,根据菌落的形态、触酶实验、革兰氏染色实验及16S r DNA鉴定及细菌表面蛋白鉴定,筛选出磷脂酰胆碱阳性表达的Aa菌株,并通过粘附侵袭实验,研究磷脂酰胆碱在Aa致病中的作用。结果侵袭性牙周炎患者的龈下菌斑通过Aa的选择性培养及鉴定,筛选出了1株磷脂酰胆碱阳性的Aa菌株,在粘附和侵袭实验中,发现磷脂酰胆碱被其特异性单克隆抗体小鼠抗磷脂酰胆碱单克隆抗体TEPC-15阻断后,磷脂酰胆碱阳性的Aa对人脐静脉血管内皮细胞的粘附率和侵袭率分别为对照组的(31.87±4.22)%和(24.63±3.55)%,粘附率(t=27.981,P=0.001)和侵袭率(t=36.786,P=0.001)与对照组相比差异均有统计学意义。结论磷脂酰胆碱有助于Aa对人脐静脉血管内皮细胞的粘附和入侵。Objective To explore the role of phosphorylcholine （ PC） during Aggregatibacter actinomycetemcomitans （ Aa） adhesion and invasion of human umbilical vein endothelial cells （ HUVEC） . Methods Non-attached plaque in periodontal pockets of 7 patients with aggressive periodontitis was collected and then inoculated on the selective medium TSBV plate. The phosphorylcholine positive expression Aa clinical strains were isolated through colony morphology, cata-lase test, Gram stain experiment, 16S rDNA and bacterial protein expression. Then, adhesion and invasion tests were taken to explore the role of phosphorylcholine during Aa pathogenisis process. Results After selective culture and spe-cific identification, one phosphorylcholine positive expression Aa clinical strain was found by Western blot. After pretrea-ted with anti-PC monoclonal antibody TEPC-15, the effect of Aa adhesion to and invasion of HUVEC was obviously de-clined （ 31. 87 ± 4. 22）% and （ 24. 63 ± 3. 55）% respectively. Conclusion PC helps PC positive Aa adhesion to and in-vasion of HUVEC.

关 键 词：伴放线放线杆菌 磷脂酰胆碱 粘附与侵袭 

分 类 号：R781.4[医药卫生—口腔医学]