肾透明细胞癌血浆miR-210、miR-378的表达及临床意义  

Expression of plasma miR-210,miR-378 and theirs clinical significance in renal clear cell carcinoma

作　　者：李东亮[1] 舒博[1] 唐双阳[1] 毕兴[1] 陈鹏[1] 

机构地区：[1]新疆医科大学附属肿瘤医院泌尿外科,乌鲁木齐830011

出　　处：《临床泌尿外科杂志》2015年第10期897-901,共5页Journal of Clinical Urology

基　　金：乌鲁木齐市科技基金(编号Y131310005)

摘　　要：目的:分析肾透明细胞癌患者和健康人群中循环miR-210和miR-378表达差异及其临床意义。方法:收集肾透明细胞癌患者和健康人群血液标本分别为22例和20例。采用实时荧光定量聚合酶链反应(Realtime PCR)检测miR-210和miR-378在肾透明细胞癌患者和健康人群血浆的表达水平,分析其表达水平与临床病理特征的关系。结果:miR-210、miR-378在肾透明细胞癌患者血浆中表达值[(1.76±1.88)、(0.21±0.19)]显著高于其健康人群中的表达值[(0.55±0.79)、(0.04±0.05)],差异有统计学意义(P<0.05)。miR-210的表达水平与肿瘤的病理分期(TNM分期)有关(P<0.05);而与患者性别和年龄、民族、肿瘤大小和病理核分级(Furman分级)无明显关系(P>0.05)。而miR-378的表达水平与患者性别和年龄、民族、肿瘤大小、病理分期和病理核分级无明显关系(P>0.05)。miR-210和miR-378在诊断肾透明细胞癌的AUC分别为0.803和0.802(P<0.05)。灵敏度分别为0.818和0.682,特异度分别为0.80和0.90。结论:血浆miR-210、miR-378与肾透明细胞癌的发生发展有关,可能成为肾透明细胞癌的筛查诊断的生物学指标。Objective：To investigate the difference of plasma miR-210 and miR-378 expression between patients with renal clear cell carcinoma and healthy people,and evaluate the clinical significance.Method：We collected blood specimens from 22 renal clear cell carcinoma patients and 20 healthy people.The level of plasma miR-210 and miR-378 were quantified by real-time reverse transcriptase polymerase chain reaction（Real-time PCR）,and the relationship between miR-210,miR-378 level and clinical and pathological factors was explored.Result：The expression of plasma miR-210 and miR-378 in renal clear cell carcinoma[（1.76±1.88）,（0.21±0.19）]was significantly higher than that in the healthy people[（0.55±0.79）,（0.04±0.05）].The difference was statistically significant（P〈 0.05）.The high expression of miR-210 was associated with pathological stage（TNM stage）（P 〈0.05）.There was no statistical difference of the expression of miR-210 among different genders,ages,nations,tumor sizes and Furman grades by the statistical analysis（P〉 0.05）.The high expression of miR-378 was not associated with gender,age,nation,tumor size,pathological stage and Furman grade by the statistical analysis（P 〉0.05）.The study show that plasma miR-210 and miR-378 have significant diagnostic value for renal clear cell carcinoma.MiR-210 showed the areas under the ROC curve was 0.803（P 〈0.05）with 81.8%in sensitivity and 80.0%in specificity,and miR-378 showed the areas under the ROC curve was 0.802（P 〈0.05）with 68.2%in sensitivity and 90.0%in specificity.Conclusion：Plasma miR-210 and miR-378 play a role in the development and progression of renal clear cell carcinoma and may be potential biomarkers in the screening diagnosis of renal clear cell carcinoma.

关 键 词：肾透明细胞癌 实时荧光定量 MIRNAS 血浆 

分 类 号：R737.11[医药卫生—肿瘤]