七氟醚、异氟醚对骨肉瘤MG63细胞增殖、凋亡及化疗敏感性的影响  

Effects of Sevoflurane and Isoflurane on Proliferation, Apoptosis and Chemosensitivity of Osteosarcoma Cell Line MG63

作　　者：邹海盯[1] 孙丽[1] 陶蕾[1] 高昌俊[1] 孙绪德[1] 

机构地区：[1]第四军医大学唐都医院麻醉科,陕西西安710038

出　　处：《现代生物医学进展》2015年第23期4419-4423,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81271343)

摘　　要：目的:观察不同浓度的七氟醚(Sev)、异氟醚(Iso)对骨肉瘤MG63细胞增殖、凋亡及对顺铂化疗敏感性的影响。方法:体外培养骨肉瘤MG63细胞。用噻唑蓝比色法(MTT法)检测不同浓度七氟醚和异氟醚对MG63细胞增殖的影响。平板克隆形成检测不同浓度七氟醚和异氟醚对MG63细胞克隆形成率的影响。流式细胞术检测细胞凋亡。结果:七氟醚、异氟醚对骨肉瘤MG63细胞增殖有一定抑制作用(P<0.05),相对其它浓度5.1%Sev和2.6%Iso抑制作用更明显(P<0.05)。七氟醚、异氟醚对骨肉瘤MG63细胞克隆形成有抑制作用(P<0.05)。不同浓度Sev、Iso促进MG63凋亡作用不明显(P>0.05)。2.5%Sev、2.0%Iso使骨肉瘤MG63细胞对顺铂诱导的凋亡率降低(P<0.05)。结论:不同浓度的七氟醚、异氟醚可抑制骨肉瘤MG63细胞的增殖及克隆形成,高浓度的七氟醚、异氟醚对其增殖抑制作用更明显。七氟醚和异氟醚不促进骨肉瘤MG63细胞的凋亡,但可以降低其对顺铂化疗敏感性。Objective： To investigate the effects of different concentrations of sevoflurane and isoflurane on proliferation,apoptosis and chemosensitivity to cisplatin of MG63 cells. Methods： MG63 cells were inoculated in culture plate. The proliferation inhibition rates, colony formation rates and apoptosis percentages of MG63 cells were measured by methyl thiazolyl tetrazolium（MTT）assay, colony formation assay and flow cytometer respectively after exposure to different concentrations of sevoflurane and isoflurane.Results： Sevoflurane and isoflurane inhibited to some extent, the proliferation of MG63 cells. Compared to that of other concentrations（P〈0.05）, the inhibition effect of 5.1% Sevoflurane and 2.6% Isoflurane was more obvious（P〈0.05）. Sevoflurane and isoflurane inhibited the colony formation of MG63 cells（P〈0.05）. Different concentrations of sevoflurane and isoflurane had no effect on apoptosis of MG63 cells（P〉0.05）. 2.5%Sevoflurane and 2.0%Isoflurane decreased the apoptosis percentages induced by cisplatin on MG63 cells. Conclusion：Different concentrations of sevoflurane and isoflurane can inhibit the proliferation and colony formation of MG63 cells, and it is more obvious to the proliferation inhibition rates of MG63 cells by high concentrations of Sevoflurane and Isoflurane. Sevoflrane and isoflurane don＇t promote the apoptosis of MG63 cells, but can reduce its chemosensitivity to cisplatin.

关 键 词：七氟醚 异氟醚 骨肉瘤 顺铂 凋亡 

分 类 号：R783.1[医药卫生—口腔医学]