TRF1、TRF2和POT1基因mRNA在前列腺癌组织中的表达  

Expressions of TRF1,TRF2 and POT1 Gene mRNA in Prostate Cancer

作　　者：王英磊[1] 逄淑超[2] 张莹莹[3] 李忠海[1] 李磊[4] 孟琳[1] 肖琳[1] 

机构地区：[1]济宁医学院附属医院泌尿外科,山东济宁272000 [2]济宁医学院附属医院检验科,山东济宁272000 [3]济宁医学院附属医院小儿科,山东济宁272000 [4]济宁医学院附属医院病理科,山东济宁272000

出　　处：《现代生物医学进展》2015年第23期4518-4520,4524,共4页Progress in Modern Biomedicine

基　　金：济宁市科技计划项目(2014jnjc15)

摘　　要：目的:探究端粒重复序列结合蛋白质1(TRF1)、TRF2和端粒保护蛋白(POT1)基因mRNA在前列腺癌(PCa)组织中的表达。方法:收集46例PCa患者肿瘤中心组织(中心组织组)和35例前列腺增生(BPH)患者BPH组织(BPH组织组),提取总RNA,逆转录成cDNA,采用定量PCR测定TRF1、TRF2和POT1在中心组织组和BPH组织基因mRNA的表达,采用基因mRNA所得CT值与β-actin mRNA所得CT值之差(△CT值)表示,并进行差异性分析。结果:中心组织组TRF1的△CT值高于BPH组织组,差异有统计学意义(Z=-3.469,P=0.001);TRF2的△CT值分别为8.49(5.75,10.21)和8.16(6.28,9.75),差异无统计学意义(Z=-1.719,P=0.086);中心组织组POT1的△CT值高于BPH组织组,差异有统计学意义(t=-18.48,P=0.000)。结论:TRF1和POT1在PCa肿瘤组织中的表达升高,说明TRF1和POT1的高表达可能与PCa的发生和发展有关,但TRF2在PCa肿瘤中心组织和BPH组织中的表达没有表现出差异,有待进一步研究。Objective： To explore the expression of telomeric repeat binding factor 1（TRF1）, factor 2（TRF2） and protection of telomeres1（POT1） gene mRNA in prostate cancer（PCa）. Methods： A total of 46 cases of PCa center tissues（as center tissue group）, and35 cases of benign prostatic hyperplasia（BPH） tissues（as BPH tissue group） were collected and extracted the total RNA and transcribed into cDNA in reverse. The gene mRNA expressions of TRF1, TRF2 and POT1 were detected by quantitative PCR method. The difference（△CT value） between the value of CT of gene m RNA and the CT value of beta-actin m RNA was analysed. Results： The△CT value of TRF1 in center tissue group was higher than that of BPH tissue group, and the difference was statistically significant（Z=-3.469, P=0.001）. The △CT values of TRF2 in center tissue group and BPH tissue group were 8.49（5.75, 10.21） and 8.16（6.28, 9.75）, but the difference was not statistically significant（Z=-1.719, P=0.086）. The△CT value of POT1 in center tissue group was higher than that of BPH tissue group, and the difference was statistically significant（t=-18.48, P=0.000）. Conclusion： The high expression of TRF1 and POT1 in PCa center tissue may be associated with the development and progression of PCa, but TRF2 shows no difference in PCa center tissue and BPH tissue, which remains to be further studied.

关 键 词：前列腺癌 端粒重复序列结合蛋白质1 端粒重复序列结合蛋白质2 端粒保护蛋白1 

分 类 号：R737.25[医药卫生—肿瘤]