抗肿瘤坏死因子-α单克隆抗体对脑缺血再灌注大鼠的抗氧化作用  

Antioxidation effect by anti-tumor necrosis factor-α monoclonal antibody for cerebral ischemiareperfusion rats

作　　者：陈金 吴丽娥[2] 

机构地区：[1]内蒙古自治区人民医院神经内科,010017 [2]包头医学院第一附属医院神经内科

出　　处：《中国实用医药》2015年第27期282-283,共2页China Practical Medicine

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)单克隆抗体对脑缺血再灌注大鼠的抗氧化作用。方法 76只健康雌性SD大鼠,随机分为假手术组(4只)、抗体组(36只)和生理盐水组(36只),采用Zea Longa线栓法建立局灶性脑缺血再灌注模型,测定大鼠血清丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果 TNF-α单克隆抗体在再灌注3、6 h后可降低血清中MDA含量,提高SOD活性。结论抗TNF-α单克隆抗体在一定时间内能提高脑缺血再灌注大鼠的抗氧化作用。Objective To investigate antioxidation effect by tumor necrosis factor-α（TNF-α） monoclonal antibody for cerebral ischemia-reperfusion rats. Methods A total of 76 health male SD rats were randomly divided into sham operation group（4 cases）, antibody group（36 ases）, and normal saline group（36 cases）. Model of focal cerebral ischemia-reperfusion rats was established by Zea Longa line plug method.Serum malondialdehyde（MDA） content and superoxide dismutase（SOD） activity were detected. Results 3 and 6 h of TNF-α monoclonal antibody reperfusion reduced serum MDA content and improvded SOD activity. Conclusion Anti-TNF-αmonoclonal antibody can improve antioxidation effect in cerebral ischemiareperfusion rats for a certain time.

关 键 词：抗肿瘤坏死因子-Α单克隆抗体 脑缺血再灌注损伤 丙二醛 超氧化物歧化酶 

分 类 号：R743[医药卫生—神经病学与精神病学]