miR-15b对口腔鳞癌细胞Cal27增殖与凋亡能力影响研究  

Influence of miR-15b on Cal27 proliferation and apoptosis in oral squamous cell carcinoma

作　　者：郭艳[1] 尚超[2] 任美思[3] 宋荣博[1] 钟鸣[1,3] 

机构地区：[1]中国医科大学附属口腔医院辽宁省口腔医学重点实验室,沈阳110002 [2]中国医科大学基础医学院神经生物教研室,沈阳110002 [3]中国医科大学附属口腔医院口腔组织病理学教研室,沈阳110002

出　　处：《中国实用口腔科杂志》2015年第8期458-460,共3页Chinese Journal of Practical Stomatology

基　　金：国家自然科学基金(81301834);辽宁省自然科学基金(2013021094)

摘　　要：目的研究口腔鳞癌(OSCC)中miR-15b的表达及其对OSCC Cal27细胞增殖和凋亡能力的影响。方法实时定量PCR检测2013年1月至2015年1月期间中国医科大学附属口腔医院外科50例住院患者OSCC组织中mi R-15b基因的表达水平。培养OSCC Cal27细胞,将mi R-15b的前体转染Cal27细胞,MTT法及双标流式法检测转染前后Cal27细胞的增殖和凋亡能力。结果与癌旁组织相比,mi R-15b在OSCC组织中的表达显著下调,且与OSCC的分级相关。mi R-15b的前体能够使Cal27细胞的增殖能力明显下降,且能够诱导Cal27细胞发生凋亡。结论 mi R-15b的异常表达与OSCC相关,mi R-15b能够抑制OSCC细胞增殖并诱导其凋亡,在OSCC中发挥肿瘤抑制基因的作用。Objective To explore the expression of miR-15b in oral squamous cell carcinoma（OSCC） and the influence of miR-15b on OSCC Ca127 proliferation and apoptosis. Methods Real-time PCR was used to detect the expression of miR-15b in OSCC and its adjacent tissues. The Ca127 cells were cultured and transfeeted with miR-15b precursor, then the proliferation and apoptosis ability of Ca127 cells were detected by MTT assay and double labeling flow cytometry method. Results The miR-15b was down-regulated in OSCC tissues compared with the adjacent tissues and its expres- sion showed a negative relationship with the grades of OSCC. The miR-15b precursor could significantly inhibit the Ca127 cells proliferation and induce the Ca127 cells apoptosis. Conclusion The abnormal expression of miR-15b is in- volved in OSCC; miR-15b could inhibit the proliferation ability and induce apoptosis ability of Ca127 ceils. The miR-15b acts as a tumor suppressor in OSCC.

关 键 词：口腔鳞癌 增殖 凋亡 miR-15b 

分 类 号：R78[医药卫生—口腔医学]