大洋纵列海鞘共附生链霉菌Streptomyces sp. FDZ35-2的化学成分  

Secondary metabolites of Streptomyces sp. FDZ35-2 associated with Symplegma oceania

作　　者：梅东海[1,2] 田晓清[1] 樊成奇[1] 赵树明[1] 杨桥[1] 马丽艳[1] 黄洪亮[1] 陆亚男[1] 

机构地区：[1]农业部东海与远洋渔业资源开发利用重点实验室农业部东海与长江口渔业资源环境科学观测实验站中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学,上海201306

出　　处：《海洋渔业》2015年第5期473-478,共6页Marine Fisheries

基　　金：上海市自然科学基金(No.13ZR1450000);农业部海洋渔业可持续发展重点实验室开放课题(No.2013-SDMFMA-KF-1);南极周边海域磷虾等生物资源考察与评估专项(No.CHINARE2015-01-06)

摘　　要：海鞘共附生微生物是尚待关注的海洋微生物资源重要来源之一。本文研究了大洋纵列海鞘（Symplegma oceania）的一株共附生链霉菌Streptomyces sp. FDZ35-2的代谢产物,以探讨其主要代谢产物的结构类型和菌株的潜在药用价值。运用多种柱层析和HPLC等分离技术,从菌株甲醇提取物中分离鉴定2个主要的单体化合物;经LC-MS、1H-和13C-NMR等波谱学方法和文献数据对照,鉴定化合物结构分别为：9-甲基-β-咔啉-3-羧酸甲酯（1）和放线菌素D（2）。考虑放线菌素D生物合成可能利用培养基氮源中的氨基酸,本文还利用磷虾蛋白水解产物代替商品蛋白胨作为培养基氮源,对Streptomyces sp. FDZ35-2的发酵条件进行了初步研究,结果显示能产出更多种放线菌素D的结构类似物的发酵培养基为：磷虾蛋白水解产物2.0 g、葡萄糖2.0 g、硫酸铵1.0 g、碳酸钙0.5 g、氯化钠0.05 g、磷酸二氢钾0.05 g、海水1.0 L,p H 7.8~8.0。对比实验结果表明：与商品蛋白胨组相比,南极磷虾蛋白水解产物作为发酵培养基氮源所发酵产生的放线菌素D的结构类似物多样性更好,二者产生总放线菌素含量相当。这为开发南极磷虾蛋白在微生物发酵工业中的新用途提供了依据。Ascidian-derived microbes are important marine microorganism resources that need more attention. Investigation on secondary metabolites of Streptomyces sp. FDZ35 - 2, associated with ascidian Symplegma oceania, has been carried out to understand its chemical constituent types and its potential in medicinal development. Two compounds were obtained by various column chromatography and preparative HPLC, and their structures were identified as 9-methyl-β-earboline-3-earboxylate （1） and actinomyein D （2） by comparison of LC-MS, 1 H- and 13C-NMR data with those reported. The amino acids of N-sources in culture medium may be applied in biosynthesis of actinomycin D. Therefore, Antarctic krill protein hydrolysate was used as N-sources in culture medium, and the fermentation conditions of Streptomyces sp. FDZ35 - 2 were optimized by analysis of its metabolites compared with commercially obtained peptones. To yield more actinomyein D derivatives, better fermentation medium for this strain in 1.0 L sea water should contain krill protein hydrolysate 2.0 g, glucose 2.0 g, ammonium sulfate 1.0 g, calcium carbonate 0. 5 g, sodium chloride 0.05 g, and potassium dihydrogen phosphate 0. 05 g. The pH value should be 7.8 - 8.0. The results of comparative experiments showed that the Antarctic krill protein hydrolysate was better than some peptones in the market as N-sources in fermentation mediums to produce various actinomycin D derivatives, and the total actinomycins yields were close. This research may be a good basis for developing a new application of Antarctic krin protein as peptone material in the microorganisms fermentation industry.

关 键 词：共附生微生物 大洋纵列海鞘 次级代谢产物 放线菌素D 南极磷虾蛋白 

分 类 号：S986.3[农业科学—捕捞与储运]