稳定性同位素示踪技术的蛋白质代谢动力学研究  

Use of stable isotope tracers for studies of protein metabolic kinetics

作　　者：周波[1,2] 任建安[1] 

机构地区：[1]南京军区南京总医院解放军普通外科研究所,江苏南京210002 [2]安徽医科大学第一附属医院普通外科,安徽合肥230022

出　　处：《肠外与肠内营养》2015年第5期300-303,共4页Parenteral & Enteral Nutrition

基　　金：国家自然科学基金资助项目(81270478)

摘　　要：同位素示踪技术是公认的用于研究蛋白质代谢动力学的最佳方法。起初,由于放射性同位素具有方便、经济、检测技术成熟等优点而得到广泛应用,但因它的放射性限制了在人体代谢研究中的应用。随着稳定性同位素标记技术的成熟和质谱分析技术的发展和普及,稳定性同位素被广泛用于人体代谢动力学研究中。以下就稳定性同位素用于研究蛋白质代谢动力学中的基本原理、方法、检测手段和应用作一综述。It is well known that the tracer technique remains the optimal method for studies of metabolic kinetics. Initially, radioactive isotope was widely used due to its convenience, economy, and accurate measurement. However, concern over waste disposal and radiation exposure for human subjects limited its application in human studies. Recent improvements in stable isotope tracer technique, as well as mass spectrometric analysis, have increased the popularity and reliability of stable isotopic enrichment measurements. The approaches used in the application of stable isotope tracers to studies of protein meta- bolic kinetics and the principles and technical issues regarding mass spectrometry were reviewed.

关 键 词：稳定性同位素 蛋白质转换 合成速率 质谱 营养代谢 

分 类 号：R459.3[医药卫生—治疗学]