雷公藤单体抑制结缔组织生长因子对脑胶质瘤细胞增殖的影响  

Role of triptolide / tripterine in glioma cell proliferation induced by connective tissue growth factor

作　　者：马春阳[1,2] 万小平[1] 杨堃[2] 王子玲[2] 郑传宜[2] 叶富跃[2] 吴然[2] 

机构地区：[1]中南大学湘雅医院肝胆肠外科,长沙410008 [2]海南医学院附属医院神经外科,海口570102

出　　处：《中国临床药理学杂志》2015年第19期1952-1954,共3页The Chinese Journal of Clinical Pharmacology

基　　金：2014年海南省社会发展科技专项基金资助项目(SF201407);2015年海南省中药现代化专项基金资助项目(2015ZY23);海南省卫生厅科研立项基金资助项目(2009-44;2006-56)

摘　　要：目的探讨结缔组织生长因子(CTGF)对脑胶质瘤细胞增殖的影响,并分析Rho激酶信号转导通路及雷公藤单体在其中的作用。方法取传代培养生长的脑胶质瘤细胞,按如下进行操作:正常对照组未予以任何无药物干预,CTGF组予以2.5μg·L-1CTGF孵育细胞24 h;雷公藤甲素组予以2.5μg·L-1CTGF与0.5 ng·m L-1雷公藤甲素共同孵育细胞24 h;雷公藤红素组予以2.5μg·L-1CTGF与0.5 ng·m L-1雷公藤红素共同孵育细胞24 h;Y27632(Rho激酶特异性抑制剂)组予以1μmol·L-1Y27632预处理细胞30 min后,加入2.5μg·L-1CTGF作用24 h。用酶联免疫吸附测定法对Rho激酶活性进行检测。用氚化胸腺嘧啶核苷法检测脑胶质瘤细胞的增殖。结果 CTGF组脑胶质瘤细胞DNA的合成量显著高于正常对照组(P<0.01),雷公藤甲素组和雷公藤红素组脑胶质瘤细胞DNA的合成量显著低于CTGF组(P<0.05),Y27632组脑胶质瘤细胞DNA的合成量显著低于CTGF组(P<0.05)。CTGF组的Rho激酶活性显著高于正常对照组(P<0.01),雷公藤甲素组和雷公藤红素组的Rho激酶活性显著低于CTGF组(P<0.05)。结论 CTGF可诱导脑胶质瘤细胞的增殖,雷公藤甲素和雷公藤红素可抑制CTGF诱导的脑胶质瘤细胞的增殖,Rho激酶信号转导通路参与CTGF诱导的脑胶质瘤细胞增殖。Objective To explore the role of Rho- kinase in glioma cell proliferation induced by connective tissue growth factor（ CTGF） and effects of triptolide / tripterine. Methods A subculture growth of glioma cells were got to take the experiment,the normal control group was not give any drug intervention. CTGF group was given CTGF 2. 5 μg ·L^-1and incubation for 24 h. Triptolide group was given CTGF 2. 5 μg·L^-1and triptolide 0. 5 ng · m L^-1and incubation for 24 h. Tripterine group were given CTGF 2. 5 μg·L^-1and tripterine 0. 5 ng·m L^-1and incubation for 24 h. Y27632（ group specificity of Rho kinase inhibitor） cells after 30 min to give CTGF 2. 5 μg·L^-1and Y27632 1 μmol·L^-1pretreatment. The expressions of Rho- kinase protein were detected by ELISA. Glioma cell proliferation was measured by3H- TdR assay.Results CTGF could induce the proliferation of glioma cell. Triptolide,tripterine and Y27632（ Rho kinase inhibitor） could significantly reverse these effects（ P〈0. 05）. In addition,CTGF could induce the activationof Rho- kinase（ P〈0. 01）,while triptolide and tripterine could significantly reverse these effects（ P〈0. 05）,indicating the activation of Rho- kinase pathway participates in glioma cell proliferation induced by CTGF.Conclusion CTGF could induce the proliferation of glioma cell,while triptolide,tripterine and Y27632 could significantly reverse these effects. Rho- kinase pathway participates in glioma cell proliferation induced by CTGF.

关 键 词：雷公藤甲素/红素 脑胶质瘤细胞 结缔组织生长因子 RHO激酶 

分 类 号：R739.4[医药卫生—肿瘤] R285.5[医药卫生—临床医学]