抗氧化酶系统异常导致衰老骨髓间充质干细胞的成骨分化能力下降  

Dysfunction of enzymatic antioxidant system inhibits osteogenic differentiation of aged bonemarrow mesenchymal stem cells

作　　者：廖立 苏晓霞[1,2] 金岩 

机构地区：[1]第四军医大学口腔医院组织工程研究中心军事口腔医学国家重点实验室,陕西西安710032 [2]西安交通大学医学院附属口腔医院正畸科,陕西西安710004

出　　处：《口腔生物医学》2015年第3期113-117,共5页Oral Biomedicine

基　　金：国家重点基础研究发展计划(973计划)(2011CB964700)

摘　　要：目的:研究衰老骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)的氧化应激水平,以及氧自由基(reactive oxygen species,ROS)对其成骨分化的影响,探索ROS升高的分子机制。方法:通过碱性磷酸酶(alkaline phosphatase,ALP)染色及实时定量RT-PCR比较年轻及衰老BMSCs成骨分化能力,利用荧光显微镜及流式细胞术检测BMSCs中ROS水平,并检测超氧化物歧化酶2(superoxide dismutase2,SOD2)及过氧化氢酶(catalase,CAT)在不同BMSCs中的表达水平。结果:发现衰老BMSCs的成骨分化能力较年轻BMSCs显著下降,衰老BMSCs内ROS水平升高是导致其成骨分化下降的重要因素;SOD2及CAT介导的抗氧化机制异常导致ROS上升。结论:抗氧化酶表达下降引起衰老BMSCs内ROS水平升高,抑制其成骨分化。Objective：To compare the reactive oxygen species （ROS） ieveis in young and aged bone marrow mesenchymai stem ceiis（BMSCs）,expiore whether redundant reactive oxygen species （ROS） inhibits osteogenic differentiation of aged BMSCs ,and inves-tigate the mechanism of ROS enhancement .Methods：Osteogenic differentiation of BMSCs was confirmed by ALP staining and reaitime RT-PCR.Fiuorescence anaiysis and Fiow cytometry were performed to detect ROS ieveis .Reaitime RT-PCR was performed to measure superoxide dismutase 2（SOD2） and cataiase（CAT） expression.Results：Osteogenic differentiation of aged BMSCs was significantiy de-creased compared to young BMSCs .Redundant ROS inhibited osteogenic differentiation of aged BMSCs .ROS ieveis in aged BMSCs were enhanced by dysfunction of SOD2 and CAT.Conclusions：Dysfunction of enzymatic antioxidant system ieads to oxidative damage ,which inhibits osteogenic differentiation of aged BMSCs.

关 键 词：骨髓间充质干细胞 衰老 氧化应激 细胞分化 

分 类 号：R780.2[医药卫生—口腔医学]