组织特异性间充质干细胞的牙周再生对比  

Comparison of specific-tissue derived mesenchymal stem cells in periodontal regeneration

作　　者：朱斌[1,2] 赵喜聪[1] 李楠[2] 金岩[1] 

机构地区：[1]第四军医大学组织工程与再生中心军事口腔医学国家重点实验室,陕西西安710032 [2]西藏军区总医院口腔科,西藏拉萨850007

出　　处：《口腔生物医学》2015年第3期150-154,共5页Oral Biomedicine

摘　　要：目的:对比不同特异性组织来源的间充质干细胞的牙周再生能力。方法:体外培养人牙周膜干细胞(human periodontal ligament stem cells,h PDLSCs),人颌骨来源的骨髓间充质干细胞(human jaw-derived bone marrow-derived mesenchymal stem cells,h JBMMSCs)和人髂骨来源的骨髓间充质干细胞(human iliac-derived BMMSCs,h IBMMSCs),成骨诱导后进行成骨指标检测。间接共培养JBMMSCs/IBMMSCs与PDLSCs,通过RT-PCR检测成骨相关基因,观察组织特异性来源的细胞间交互作用。构建JBMMSCs/IBMMSCs+PDLSCs细胞聚合体(cell aggregates,CAs),检测成骨基因表达以及裸鼠皮下8 w异位牙周再生能力。结果:经成骨诱导,h JBMMSCs的成骨相关指标表达均显著高于h IBMMSCs和h PDLSCs(P<0.05)。经JBMMSCs诱导的PDLSCs的成骨基因表达显著高于IBMMSCs(P<0.05);JBMMSCs+PDLSCs CAs成骨相关基因表达高于IBMMSCs+PDLSCs CAs(P<0.05),并且在裸鼠皮下异位再生牙周组织,前者形成排列规律的垂直于CBB表面的牙周膜样纤维,并且有更多新生骨基质沉淀。结论:PDLSCs和JBMMSCs是具有组织特异性的间充质干细胞,是更合适牙周组织再生的种子细胞。Objective：To compare the periodontai regeneration of different specific-tissue derived mesenchymai stem ceiis .Meth-ods：Human periodontai iigament stem ceiis （ hPDLSCs ） , human jaw-derived bone marrow-derived mesenchymai stem ceiis （hJBMMSCs） and human iiiac-derived bone marrow-derived mesenchymai stem ceiis（hJBMMSCs） were cuitured in vitro.After osteo-genic induction ,reiative osteogenic tests were performed .PDLSCs induced by JBMMSCs/IBMMSCs were tested for osteogenic expression by RTPCR.Ceii aggregates （CAs） of PDLSCs and JBMMSCs/IBMMSCs were compound.The osteogenic genes expression were tested;PDL regeneration in nude mice ectopic transpiantation was observed .Results：After osteogenic induction , reiative osteogenic tests of JBMMSCs were higher than those of IBMMSCs and PDLSCs .PDLSCs induced by JBMMSCs expressed stronger osteogenic genes than those by IBMMSCs .CAs of PDLSCs/JBMMSCs expressed stronger osteogenic genes ,made more reguiar and verticai inserting fibers into the surface of CBB,regenerated more new bone matrix than CAs of PDLSCs /IBMMSCs.Conclusions：JBMMSCs and PDLSCs are spe-cific-tissue MSCs and more appropriate for PDL regeneration .

关 键 词：牙周再生 颌骨骨髓间充质干细胞 髂骨骨髓间充质干细胞 牙周膜干细胞 细胞聚合体 再生微环境 

分 类 号：R780.2[医药卫生—口腔医学]