何首乌及其成分对人正常肝细胞凋亡的影响  

Effects of Polygoni Multiflori Radix and Its Three Ingredients on Human Normal Hepatocyte Apoptosis

作　　者：贾歌刘畅 庞晶瑶 柏兆方[2] 牛明[2] 赵艳玲[2] 朱云[3] 张雅铭[2] 马致洁[1] 赵奎君[1] 王伽伯[2] 肖小河[3] 

机构地区：[1]首都医科大学附属北京友谊医院,北京100050 [2]解放军第302医院全军中医药研究所,北京100039 [3]解放军第302医院中西医结合肝病诊疗与研究中心,北京100039

出　　处：《中国中医药信息杂志》2015年第11期46-49,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81373984);国家科技重大专项-重大新药创制(2015ZX09501-004-001-008);中医药行业科研专项(201507004-04)

摘　　要：目的观察何首乌对人正常肝细胞的毒性和细胞凋亡的影响,探讨何首乌致肝损伤的可能物质和途径。方法以人正常肝(实质)细胞系L02为评价模型,Giemsa染色观察给药后各组细胞形态,流式细胞术检测给药后各组细胞凋亡率及细胞周期。结果与空白对照组比较,何首乌70%乙醇提取物1、2、4 mg原药材/m L对肝细胞形态和细胞周期影响均不明显,给药24 h凋亡率分别为9.51%、10.3%、13.2%;何首乌中3个成分大黄素、大黄酸和没食子酸(25、50、100μmol/L)单独给药,各给药组细胞均可见典型凋亡形态,G1期细胞所占比例明显减小,S期、G2期比例增加,其中3个成分高浓度(100μmol/L)给药24 h凋亡率分别为19.8%、12.0%和13.7%。结论何首乌对人L02细胞有一定的诱导凋亡作用,其所含大黄素诱导凋亡作用强于大黄酸和没食子酸。Objective To observe the effects of Polygoni Multiflori Radix on toxicity and apoptosis of human normal hepatocyte;To discuss the possible substances and channels of Polygoni Multiflori Radix to hepatic injury. Methods L02 cells were set as evaluation model. Cellular morphology in each group after medication was observed through Giemsa dyeing. Apoptosis rate and cell cycle of each group after medication were detected by FCM. Results Compared with blank control group, the 70% alcohol extracts of Polygoni Multiflori Radix(1, 2 and 4 mg raw materials per m L) had almost no effects on cellular morphology or cell cycle of L02 cells. Apoptosis rates after treatment with extracts of Polygoni Multiflori Radix were 9.51%, 10.3%, and 13.2% respectively. When L02 cells were treated with different dosages of emodin, rhein and gallic acid, three ingredients of Polygoni Multiflori Radix, apoptosis was obvious. The proportion of G1 phase cells significantly decreased, the S phase and G2 phase increased, which presented the concentration dependent effect. Apoptosis rates of three high dosages(100 μmol/L) treated groups after treatment with emodin, rhein and gallic acid were 19.8%, 12.0%, and 13.7% respectively. Conclusion Polygoni Multiflori Radix has apoptosis-inducing effects on L02 cells. Apoptosis-inducing effects of its ingredient of emodin is stronger than rhein and gallic acid.

关 键 词：何首乌 大黄素 大黄酸 没食子酸 肝细胞 凋亡 细胞周期 

分 类 号：R285.5[医药卫生—中药学]