7-二氟甲氧基-5,4'-二正辛烷氧基金雀异黄素对人卵巢癌裸鼠移植瘤生长的影响  

Effects of 7-difluoromethoxyl-5,4'-di-n-octylygenistein on the Growth of Human Ovarian Carcinoma Xenografts in Nude Mice

作　　者：陈砚芬[1] 白军[2] 徐家丽[1] 宋晓晖[1] 宁映霞[3] 

机构地区：[1]武汉市妇女儿童医疗保健中心妇产科,湖北武汉430000 [2]深圳市龙岗区妇幼保健院妇产科,广东深圳518172 [3]广州医科大学附属第一医院妇科,广东广州510120

出　　处：《现代生物医学进展》2015年第22期4239-4243,共5页Progress in Modern Biomedicine

基　　金：国家自然科学青年基金项目(81301894)

摘　　要：目的:研究7-二氟甲氧基-5,4'-二正辛烷氧基金雀异黄素(7-difluoromethoxyl-5,4'-di-n-octylygenistein,DFOG)对人卵巢癌裸鼠移植瘤生长的影响。方法:建立人卵巢癌HO-8910细胞裸鼠皮下移植瘤,随机分为5组,每组5只,即生理盐水组(Saline),紫杉醇组(TAX),DFOG此处为3组(5、10、20 mg/kg)组,观察各组移植瘤的体积和重量变化,观察各组荷瘤裸鼠重量及其血清乳酸脱氢酶(LDH)、谷丙转氨酶(ALT)、肌酐值(Cr)、外周血白细胞计数(WBC)的变化。FCM检测移植瘤组织细胞的凋亡率,Western blot检测移植瘤组织细胞cortactin蛋白的表达变化。结果:DFOG各组显著抑制人卵巢癌HO-8910细胞裸鼠移植瘤生长,与Saline组比较,移植瘤体积明显减小(P均<0.05),其中DFOG10mg/kg组对移植瘤抑制率与TAX组相当(P>0.05);与Saline组比较,DFOG各组移植瘤重量明显减轻(P均<0.05),其中DFOG10mg/kg组对移植瘤重量减少率与TAX组相当(P>0.05)。与Saline组及DFOG各组比较,TAX组荷瘤裸鼠重量显著降低(P均<0.05),而Saline组和DFOG各组之间荷瘤裸鼠重量比较差异无统计学意义(P>0.05);与Saline组及DFOG各组比较,TAX组荷瘤裸鼠血清LDH、ALT、Cr值和外周血WBC计数显著降低(P均>0.05),而Saline组与DFOG各组比较,荷瘤裸鼠血清LDH、ALT、Cr值和外周血WBC计数无明显差异(P>0.05)。DFOG各组作用HO-8910细胞移植瘤16 d后,HO-8910细胞发生凋亡,与Saline组比较(P均<0.05),其中DFOG10 mg/kg组凋亡率与TAX组相当(P>0.05);同时cortactin蛋白表达降低,与Saline组比较(P均<0.05),其中DFOG10 mg/kg组凋亡率与TAX组相当(P>0.05)。结论:DFOG能抑制卵巢癌HO-8910细胞裸鼠移植瘤生长,下调cortactin蛋白表达和诱导HO-8910细胞凋亡。ABSTRACT Objective： The aim of this study is to investigate the effects of 7-difluoromethoxyl-5, 4＇-di-n-octylygenistein（DFOG） on the growth of human ovarian carcinomaHO-8910 cells xenografts in nude mice. Methods： Models of human ovarian cancer HO-8910 cells xenografts transplanted subcutaneously in nude mice were established and randomly divided into five groups （each group including five nude mice）： Saline group, Taxol group, DFOG 5 mg/kg, DFOG 10 mg/kg and DFOG 20 mg/kg, the volume and weight of Xenograts were observed and compared. The alteration of the weight of nude mice, and the change of serum levels of lactate dehydrogenase （LDH）, alanine aminotransferase （ALT）, creatinine （Cr） and peripheral blood leukocyte counts （WBC counts） were examined and compared. The cellular apoptotic rate of human ovarian carcinoma HO-8910 cells xenografts was analyzed by FCM. The expressions of cortactin protein of HO-8910 cells xenografts were determined by western blot. Results： All DFOG groups could significantly suppress the increasing volume of human ovarian carcinoma HO-8910 cells xenografts in nude mice models,there was dramatically statistical mean compared with Saline group （Paverage 〈0.05）, and the inhibiting rate of DFOG10mg/kg was similar with TAX group （P 〉0.05）. Compared with Saline group, the weight ofXenograts of DFOG groups dramatically decreased （Paverage〈0.05）, and the inhibiting rate of DFOG10mg/kg was almost equal to TAX group （P 〉0.05）. Compared with Saline group and each DFOG groups, the weight of experimental nude mice of TAX group obviously decreased （Paverage 〈0.05）, while there was no statistical significance among Saline group and three DFOG groups （P〉0.05）. Compared with Saline group and each DFOG groups, the serum levels ofLDH, ALT, Cr and WBC counts of experimental nude mice of TAX group significantly descended （Paverage ~0.05）, while there was no difference among Saline group and three DFOG groups （P 〉0.05）. When t

关 键 词：卵巢癌 7-二氟甲氧基-5 4'-二正辛烷氧基金雀异黄素 移植瘤 凋亡 

分 类 号：Q953[生物学—动物学] R737.31[医药卫生—肿瘤]