14-3-3theta蛋白在人骨肉瘤中的表达和临床意义  

Expression and Clinical Significance of 14-3-3theta Protein in Human Osteosarcoma

作　　者：杨新萍[1] 杨娜[1] 张倞[1] 刘宏[1] 银存举[1] 

机构地区：[1]北京军区总医院二六三临床部,北京101149

出　　处：《现代生物医学进展》2015年第27期5221-5224,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30770823)

摘　　要：目的:研究14-3-3theta蛋白在人骨肉瘤中的表达和临床意义。方法:在62例人骨肉瘤和正常骨组织样本中,通过免疫组化的方法检测14-3-3theta蛋白的表达情况,并分析其表达与骨肉瘤临床病理学特征间的关系。结果:在62例骨肉瘤和正常骨组织中,14-3-3theta蛋白表达的阳性率分别为80.6%和17.7%。统计学分析表明,14-3-3theta蛋白的表达与骨肉瘤肿瘤大小(P=0.016)、高组织学类型(P=0.001)、高Enneking分级(P=0.047)具有显著相关性,而与骨肉瘤患者的年龄(P=0.901)、性别(P=0.691)、肿瘤部位(P=0.802)、碱性磷酸酶(P=0.884)、血清白蛋白(P=0.822)、血沉(P=0.836)等均无关。Spearman相关性分析检测发现,14-3-3theta蛋白高表达与肿瘤大小(r=-0.34,P=0.034)、高组织学分级(r=-0.27,P=0.031)、高Enneking分级(r=-0.05,P=0.025)呈正相关,而与骨肉瘤患者的年龄、性别、肿瘤部位、碱性磷酸酶和血沉等均无相关性(P>0.05)。结论:在骨肉瘤组织中,14-3-3theta作为癌基因表达增加,与骨肉瘤的疾病进展密切相关,14-3-3theta可以作为骨肉瘤疾病监测的一种分子指标。Objective： To investigate the expression and clinical significance of 14-3-3theta protein in human osteosarcoma.Methods： Expression status of 14-3-3theta was detected by immunohistochemistry in 62 cases of human osteosarcoma and normal bone tissues. The correlation between the expression of 14-3-3theta and clinicopathological characteristics of osteosarcoma were analyzed.Results： In 62 cases of osteosarcoma and normal bone tissues, the positive expressions of 14-3-3theta were 80.6% and 17.7%. The chi square test suggested that 14-3-3theta expression was correlated with tumor size（P=0.016）, histologic subtype（P=0.001）, Enneking stage（P=0.047）,while it was not correlated with age（P=0.901）, sex（P=0.691）, site of primary disease（P=0.802）, alkaline phosphatase（P=0.884）, serum albumin（P=0.822）, ESR（P=0.836）. Correlation analysis suggested that 14-3-3theta expressions were positively correlated with tumor size（r=-0.34, P=0.034）, histologic subtype（r=-0.27, P=0.031）, Enneking stage（r=-0.05, P=0.025）, while it was negatively correlated with age, sex, site, alkaline phosphatase, serum albumin and ESR（P〉0.05）. Conclusion： In human osteosarcoma,expressions of 14-3-3theta were increased and correlated with human osteosarcoma progression. 14-3-3theta may serve as a candidate biomarker in human osteosarcoma.

关 键 词：骨肉瘤 14-3-3theta 免疫组化 临床意义 

分 类 号：R738[医药卫生—肿瘤]