野菊花提取物对牙龈卟啉单胞菌胰酶样蛋白酶活性的抑制作用  

Inhibitory effect of wild chrysanthemum extract on activity of trypsin-like protease produced by Porphyromonas gingivalis

作　　者：陈春雨[1] 刘鹤[1] 何畔[1] 卢建忠[1] 应頔 马宁[1] 

机构地区：[1]吉林大学口腔医院牙周病科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2015年第5期941-945,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省中医药管理局科研项目资助课题(2012-144)

摘　　要：目的:探讨野菊花提取物对牙龈卟啉单胞菌(P.g)胰酶样蛋白酶(TLP)活性的影响,为野菊花治疗牙周炎提供理论依据。方法:配制牛心脑浸液培养基(BHI),低、中、高浓度对倍稀释的野菊花提取物(0.1563、0.3125和0.6250g·L-1)和一定浊度的P.g菌悬液,实验分为低、中、高浓度野菊花提取物组、阳性对照组(不加野菊花提取物)和阴性对照组(加入低浓度野菊花提取物,不加P.g)。各组含溶液的EP管在37℃恒温条件下厌氧培养48h,测定培养物上清液和菌细胞破碎后上清液中TLP活性和蛋白水平,计算TLP比活。结果:与阳性对照组比较,中、高浓度野菊花提取物组上清液中TLP活性(游离态和结合态)均降低(P<0.05);与低浓度野菊花提取物组比较,中、高浓度野菊花提取物组上清液中TLP活性(游离态和结合态)均降低(P<0.05);与中浓度野菊花提取物组比较,高浓度野菊花提取物组上清液中TLP活性(游离态和结合态)降低(P<0.05),各组上清液中蛋白水平和TLP比活无明显变化(P<0.05)。结论:野菊花提取物对TLP活性有抑制作用。Objective To investigate the effect of wild chrysanthemum extract on the activity of trypsin-like protease（TLP） produced by Porphyromonas gingivalis（P.g）,and try to provide the evidence for the wild chrysanthemum extract in the treatment of periodontal disease.Methods The brain heart infusion broth（BHI）,and low,medium and high concentrations（0.1563,0.3125,and 0.6250g·L^-1）of diluted wild chrysanthemum extract and turbidity of P.gbacteria suspension were prepared.Low,medium,and high concentrations of wide chrysanthemum extract groups,positive control group（without wide chrysanthemum extract）,negative control group（with low concentration of wild chrysanthemum extract and without P.g）were set up.The Ep tube with solution in various groups were cultured under 37℃for 48 h.Then the TLP activity in the supernatant fluid of P.g and protein levels were detected.The specific activity of TLP was calculated.Results Compared with positive control group,the TLP activities in supernatant fluid in medium and high concentrations of wild chrysanthemum extract groups were decreased（P〈0.05）;compared with low concentration of wild chrysanthemum extract group,the TLP activities in supernatant fluid in medium and high concentrations of wild chrysanthemum extract groups was decreased（P〈0.05）;compared with medium concentration of wild chrysanthemum extract group,the TLP activities in supernatant fluid in high concentration of wild chrysanthemum extract group was decreased（P〈0.05）.There were no significant differences of the protein levels and TLP specific activities in supernatant fluid in various groups（P〉0.05）.Conclusion The wild chrysanthemum extract may inhibit the TLP activity.

关 键 词：野菊花 牙龈卟啉单胞菌 胰酶样蛋白酶 

分 类 号：R781.4[医药卫生—口腔医学]