磷脂酰肌醇蛋白聚糖-3在食管鳞癌中的表达及临床意义  

Expression of glypican -3 mRNA and protein in esophageal squamous cell carcinoma tissue and its clinial significance

作　　者：陈会枝[1] 李晨飞[2] 王晓慧[2] 崔圆圆[2] 刁长英 李晟磊[2] 

机构地区：[1]郑州大学第一附属医院检验科,450052 [2]郑州大学第一附属医院病理科

出　　处：《中华实验外科杂志》2015年第10期2599-2601,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81372677）;河南省基础与前沿资助项目（132300410073）

摘　　要：目的探讨食管鳞状细胞癌（ESCC）组织中磷脂酰肌醇蛋白聚糖-3（GPC-3）mRNA和蛋白的表达及意义。方法应用反转录-聚合酶链反应（RT．PCR）和免疫组织化学链霉菌抗生物素蛋白-过氧化物酶（SP）法检测50例ESCC组织、19例癌旁不典型增生组织及50例正常食管黏膜组织中GPC-3mRNA和蛋白的表达。结果在食管正常黏膜组织、癌旁不典型增生组织及癌组织中GPC-3mRNA含量依次降低，分别为0．808±0．068、0．813±0．037、0．875±0．052，组间比较差异有统计学意义（F=43．766，P〈0．05）；正常黏膜组织及癌旁不典型增生组织中GPC-3蛋白表达均低于ESCC组织，表达量分别为22．0％（11／50）、42．1％（8／19）、70．O％（35／50），组间比较差异有统计学意义（χ2=23．336，P〈0．05）；ESCC组织中GPC-3蛋白表达与癌组织的分化程度、TNM分期、浸润深度及有无淋巴结转移密切相关（P〈0．05）。结论ESCC组织中GPC-3mRNA和蛋白表达均增高，其高表达可能与ESCC发生有关。Objective To explore the expression of glypican -3 （GPC -3） mRNA and protein in esophageal squamous cell carcinoma （ESCC） tisuss. Methods The expression of GPC - 3was detected by reverse transcriptase- polymerase chain reaction （RT-PCR） and streptavid-in- peroxidase（SP） immu- nohistochemical methods in 50 cases of ESCC tissue, 19 cases of mucosa adjacent to cancer, and 50 cases of normal esophageal mucosa. Results The relative content of GPC - 3 mRNA depressed by turns in nor- mal mucosa tissue, mucosa adjacent to cancer and cancer tissue which were 0. 808 ±0. 068, 0. 813 ± 0. 037, 0. 875± 0. 052 respectively. There was a statistical significanae in group comparison （ F = 43.766, P 〈 0. 05 ）. The positive rate of GPC - 3 protein expression in normal mucosa and mucosa adjacent to canc- er were lower than that of cancer tissue which were 22.0% （11/50）, 42. 1% （8/19）, 70. 0% （35/50） re- spectively. There was a statistical significance in group comparison （3（2 =23. 336, P 〈0. 01 ）. The expres- sion of GPC - 3 protein was closely correlated with the tumor grade, TNM grade, infiltrative depth and lym- phatic metastasis in ESCC tissue （P 〈 0. 05）. Conclusion The expression of GPC - 3 mRNA and protein decreased in ESCC tissue, which suggest the GPC - 3 gene may be related to oncogenesis of ESCC tissue.

关 键 词：磷脂酰肌醇蛋白聚糖-3 食管鳞癌 反转录-聚合酶链反应 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]