废弃烟梗提取液为基质的产油脂酵母菌的筛选与鉴定  

Screening and Identification of Oleaginous Yeasts from Fermentation of Tobacco Stem Extract

作　　者：王姣[1] 陈彦好[1] 张向磊[1] 梁景辉[1] 刘慧[1] 张旭萍[1] 吴晨磊 董贵彬 丁腾阁 李彦周[2] 朱大恒[1] 席宇[1] 

机构地区：[1]郑州大学生命科学学院 [2]天昌国际烟草有限公司,河南省许昌市五一路北段461000

出　　处：《烟草科技》2015年第8期15-19,共5页Tobacco Science & Technology

基　　金：国家级大学生创新创业训练计划资助项目"废弃烟梗为基质的产油酵母菌的筛选鉴定及发酵产油优化研究"(201410459092)和"废弃烟梗固态发酵生产深红酵母的优化及扩大培养研究"(201310459009);河南省烟草公司科技项目"烟草基质有机酸发酵与资源利用生物工程"(HYKJ201125);河南省高等学校重点科研项目"干酪乳杆菌XJL发酵废弃烟梗水提取物产L-乳酸的响应面优化研究"(15B180020)

摘　　要：以废弃烟梗水提取液(Tobacco stem extract,TSE)为唯一基质,采用苏丹黑B染色法从市售鲜葡萄中初筛出12株产油脂酵母菌,进一步结合摇瓶发酵实验复筛出2株在TSE中生长良好且油脂产量较高的酵母菌CC820和CA815。对CC820和CA815的菌落、细胞及生理生化特征进行检测,CC820初步鉴定为假丝酵母(Candida sp),CA815初步鉴定为红酵母(Rhodotorula sp)。在接种量5%、装载量60/250 m L、180 r/min和30℃条件下培养5 d,菌株CC820和CA815油脂产量分别为3.53 g/L和3.29 g/L。Using tobacco stem extract （TSE） as a sole substrate, 12 strains of oleaginous yeasts were preliminarily isolated from fresh grapes by Sudan Black B staining method. Two strains, named as CC820 and CA815, with higher lipid yield and growing well in TSE were screened out by further shake flask fermentation experiments. By their colony, morphological, physiological and biochemical characteristics, CC820 was preliminarily identified as Candida sp and CA815 Rhodotorula sp. When stem was fermented at 30 ℃ for 5 days with an inoculant rate of 5%, a load capacity of 60/250 mL and 180 r/min, the maximum yield of lipid produced by CC820 and CA815 reached 3.53 g/L and 3.29 g/L, respectively.

关 键 词：烟梗 酵母菌 油脂 筛选 鉴定 

分 类 号：TS414[农业科学—烟草工业]