盐酸普萘洛尔凝胶对婴幼儿血管瘤裸鼠模型抑瘤作用的实验研究  

Experimental study on the anti-tumor effect of propranolol hydrochloride gel on infantile hemangioma model in nude mice

作　　者：孙黎波[1] 兰玉燕[2] 赵冲[3] 王忠朝[2] 范丽苑[2] 

机构地区：[1]四川泸州医学院附属口腔医院口腔颌面外科,四川泸州646000 [2]四川泸州医学院口颌面修复重建和再生实验室 [3]四川泸州医学院附属第一医院耳鼻咽喉头颈外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第20期1820-1822,1825,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨盐酸普萘洛尔凝胶治疗婴幼儿血管瘤生长的可能机制。方法:建立婴幼儿血管瘤裸鼠移植瘤模型,第45天将16只裸鼠随机分为实验组和对照组。对照组在移植后给予普通鼠食喂养;实验组在对照组基础上患处局部涂抹盐酸普萘洛尔凝胶,每天2次,持续3周。经过3周治疗后,手术切除血管瘤。采用免疫组织化学法检测病灶组织内血管内皮生长因子(VEGF)和caspase-3表达水平的变化,并采用TUNEL染色检测细胞凋亡情况。结果:与对照组比较,实验组应用盐酸普萘洛尔凝胶后血管瘤病灶内VEGF表达水平明显下降(P<0.05),caspase-3的表达及凋亡指数明显升高(P<0.05)。结论:盐酸普萘洛尔凝胶通过诱导caspase-3的表达,抑制VEGF的作用,减少血管发生,从而对婴幼儿血管瘤裸鼠模型移植瘤的生长起到抑制作用。Objective： To explore the underlying mechanisms for treatment of infantile hemangioma by propranolol hydrochloride gel. Method： Establishment of the model of infantile hemangioma xenografts in nude mice, then randomly di- vided them into two groups on the 45th day. The control group （n= 8）were given normal diet and the experimental group （n=8）additionally were applied with propranolol hydrochloride gel twice a day for 3 weeks. After 3 weeks of treatment, the hemangioma was resected. The expressions of VEGF and caspase-3 in the focal tissue were determined by immunohis- tochemistry. At the same time, the apoptosis of cell were also determined and compared using TUNEL staining technique. Result： Compared with control group, the expressions of VEGF was decreased significantly （P〈0.05）, while the expres- sions of caspase-3 and apoptosis index were increased significantly （P〈0. 05）. Conclusion： Propranolol hydrochloride gel could induce the expressions of caspase-3 and hindrance the effect of VEGF, thus to control angiogenesis, which thereby effectively inhibit infantile hemangioma xenografts in nude mice.

关 键 词：血管瘤 普萘洛尔 血管内皮生长因子 CASPASE-3 

分 类 号：R739.8[医药卫生—肿瘤]