固定矫治器对口腔菌群的影响  

The influence of fixed orthodontic appliance on oral microbiota

作　　者：刘月琴[1] 刘建均[1] 李新莉[1] 臧师竹[1] 姜涛[1] 殷玉玲[1] 

机构地区：[1]大连医科大学生物技术系,辽宁大连116044

出　　处：《中国微生态学杂志》2015年第10期1156-1159,共4页Chinese Journal of Microecology

基　　金：国家教育部回国人员科研启动基金

摘　　要：目的利用PCR-DGGE方法探讨戴用牙齿固定矫治器对口腔菌群的影响。方法从20例健康的牙齿正常个体和30例戴用固定矫治器的正畸患者的唾液中提取细菌基因组总DNA,利用聚合酶链式反应—变形梯度凝胶电泳(PCR-DGGE)方法获取口腔菌群的分子指纹图谱,对其进行相似性和多样性的分析以及优势条带的序列分析。结果 PCR-DGGE可明确将正常个体和正畸患者分成2个簇,且正畸患者口腔菌群的多样性明显增加,优势菌群发生转变,序列分析表明口腔链球菌数量明显减少,假单胞菌成为优势菌型。结论戴用固定矫治器能改变口腔菌群分布,引起口腔中有益菌的减少,促使致病菌增长,导致口腔菌群失调。Objective To study the influence of fixed orthodontic appliance on oral microbiota by PCR-DGGE （polymerase chain reaction-denaturing gradient gel electrophoresis） technique. Methods 20 subjects with healthy teeth and 30 patients with fixed orthodontic appliance were included. Bacterial genomic DNA was ex- tracted from saliva. The DGGE profile of oral microbiota was obtained with PCR-DGGE method; the diver- sity and similarity analyses as well as the sequence analysis of the dominant bands were conducted. Results The bacterial composition could be divided into two clusters among healthy people and patients with fixed or- thodontic appliance by PCR-DGGE distinctly. The bacterial richness in patients with fixed orthodontic appli ance markedly increased. Sequence analysis indicated that Streptococcus oralis was significantly reduced and Pseudomonas aynxantha became dominant organisms. Conclusion The application of fixed orthodontic ap- pliances has impact on oral microflora, reduces the amount of beneficial bacteria while increase the amount of pathogenic bacteria, and disequilibrates oral mierobiota.

关 键 词：固定矫治器 PCR-DGGE 口腔菌群 

分 类 号：R783.5[医药卫生—口腔医学]