益生菌对活动期溃疡性结肠炎大鼠结肠黏膜组织及IFN-γ、IL-12表达的影响  

Effect of Probiotic Bacteria on Colonic Mucosa Tissue and the Expression of IFN-γ and IL-12 in Rats with Ulcerative Colitis

作　　者：王岩[1] 杨立[1] 刘威羽[1] 

机构地区：[1]辽宁省人民医院消化内科,辽宁沈阳110016

出　　处：《贵阳医学院学报》2015年第11期1190-1193,共4页Journal of Guiyang Medical College

基　　金：辽宁省自然科学基金项目(201102106)

摘　　要：目的:探讨益生菌VSL#3对三硝基苯磺酸钠(TNBS)诱导的活动期溃疡性结肠炎(UC)大鼠肠道功能及干扰素(IFN)-γ、白介素12(IL-12)表达的影响。方法:21只8周龄SD大鼠,均分为对照组(A组)、TNBS模型组(B组)及VSL#3治疗组(C组),A组给予0.25 m L生理盐水灌肠,B、C组给予100 g/L TNBS 0.25 m L灌肠建立UC大鼠模型;造模第2天A、B组给予生理盐水0.25 m L灌胃,C组给予VSL#3溶液0.25 m L灌胃;第8天时处死大鼠,比较各组大鼠结肠黏膜组织形态、病理学评分、疾病活动指数(DAI)评分、髓过氧化物酶(MPO)活性变化和IFN-γ、IL-12免疫阳性细胞的表达。结果:A组肠组织未见溃疡病灶,B组肠组织可见溃疡,充血水肿程度较重,与B组比较,C组较B组溃疡减少、缩小、并有修复;光镜下A组无明显炎症细胞浸润,B组可见大量中性粒细胞及淋巴细胞浸润,黏膜下急性小血管炎症出血和纤维素样坏死,而C组较B组炎性细胞浸润减少;DAI、病理学评分C组较B组均明显下降(P<0.01),MPO活性低于B组而高于A组(P<0.05);B组IFN-γ和IL-12明显高于A组,C组较B组有降低,但仍明显高于A组(P<0.05);UC模型大鼠肠组织IFN-γ与IL-12的IOD值呈显著正相关(r=0.964 0,P<0.01),UC模型大鼠肠组织IFN-γ、IL-12的IOD值与肠组织病理学评分、MPO活性及DAI呈正相关(r=0.875 0、0.881 0及0.767 1,P<0.05;r=0.837 0、0.850 8及0.713 3,P<0.05)。结论:VSL#3辅助治疗对TNBS诱导的UC大鼠有确切疗效,其作用机制可能与益生菌VSL#3通过有效抑制大鼠结肠黏膜IFN-γ、IL-12的表达有关。Objective： To investigate the effect of probiotic VSL#3 on function and inflammatory cytokines of rats with active phase of ulcerative colitis induced by TNBS. Methods： Twenty-one adult SD rats were randomly divided into normal control group（ group A）,TNBS group（ group B）,VSL#3 treatment group（ group C）,7 in each group. Group A received enema of 0. 25 m L dose of normal saline while Group B and Group C received enema of 0. 25 m L of TNBS（ 100 g / L concentration） to construct UC rats model. After 2 days of model-building,group A and group B received intragastric administration of 0. 25 m L dose of normal saline while group C received intragastric administration of 0. 25 m L dose of VSL#3 solution. At 8thday,rats were killed. Morphologic changes of colonic mucosa tissue,pathology score,MPO activity and expression of IFN-γ,IL-12 immune positive cells were compared between group A,group B and group C. Results： There was no ulcer lesion in colonic mucosa tissue in group A while there was ulcer lesion in colonic mucosa tissue in group B,and the degree of congestion and edema was severe. Compared with group B,ulcer in group C decreased,shrank and repaired itself to some extent. Under light microscope,there existed no obvious inflammatory cell infiltration in group A while in group B there was a lot of infiltration of neutrophile granulocyte and leukomonocyte,and acute inflammation of small blood vessels and fibrinoid necrosis under mucous membrane. Compared with group B,in group C inflammatory cell infiltration decreased,pathology score decreased significantly（ P〈0. 01）,MPO activity decreased but higher than that of group A（ P〈0. 05）. IFN-γ and IL-12 expression levels in group B were significantly higher than those of group A while in group C these indexes decreased but still higher than those of group A. There were statistical significance in the differences between group A and group B,between group B and group C,and between group A and group C（ P〈0. 05）. The IOD value of IFN-

关 键 词：结肠炎 溃疡性 益生菌VSL#3 干扰素Γ 白细胞介素12 基因表达 大鼠 Sprague Dawley 

分 类 号：R574.62[医药卫生—消化系统]