假单胞菌属脂肪酶的分子生物学研究进展  

Research Advances in Molecular Biology of Pseudomonas Lipases

作　　者：查代明[1,2] 张炳火[1] 李汉全[1] 闫云君[2] 

机构地区：[1]九江学院药学与生命科学学院,九江332000 [2]华中科技大学生命科学与技术学院,武汉430074

出　　处：《中国生物工程杂志》2015年第9期114-121,共8页China Biotechnology

基　　金：深圳市创新基金(JCYJ20120831111657864);国家"863"计划(2011AA02A204)资助项目

摘　　要：微生物脂肪酶是商品化脂肪酶的主要来源,并广泛应用于诸多工业领域。与其他微生物脂肪酶相比,细菌脂肪酶催化反应的类型更多、活性更高、稳定性更好,其中又以假单胞菌属(Pseudomonas)脂肪酶的性能最为优越。作为性能最为优越、应用最为广泛的一类脂肪酶,假单胞菌属脂肪酶研究一直是脂肪酶领域的热点。就假单胞菌属脂肪酶的分子生物学研究进展进行归纳和述评,包括基因资源挖掘及克隆、基因表达调控及分泌机制、活性过表达策略、蛋白质结晶及3D结构解析、蛋白质工程,并对其未来研究方向做出展望,以期为后续研究提供有益参考。Microbial lipases,being major sources of commercial ones,which have been widely utilized in many industrial fields, such as foods, beverages, lipids, detergents, feeds, textiles, leathers, advanced materials,fine chemicals, medicines, cosmetics, papermaking, pollution treatment, and bioenergy, etc.Compared with other microbial lipases,bacterial lipases have more types of reactions and exhibit higher activity and better stability in many reactions. Amongst bacterial lipases,the most excellent ones are those being from the genus Pseudomonas. As one of the most excellent and the most widely used lipases,the study on Pseudomonas lipases has been a hot topic in the field of lipases. Research advances in molecular biology of Pseudomonas lipases,including mining and cloning of genetic resources,molecular mechanisms of gene expression regulation and protein secretion,strategies for efficient overexpression,protein crystallization and 3D structure analysis,and protein engineering,were summarized and reviewed. Furthermore,the future research directions of Pseudomonas lipases were prospected so as to provide a useful reference for the follow-up studies.

关 键 词：假单胞菌属 脂肪酶 基因表达调控 

分 类 号：Q814.9[生物学—生物工程]