玉米耐盐基因ZmHKT1的序列变异分析  

Nucleotide Diversity of Drought Tolerance Related Gene ZmHKT1

作　　者：姜志磊[1] 姜昱[1] 刘艳芝[1] 蒋超[2] 金峰学[1] 李毅丹[1] 

机构地区：[1]吉林省农业科学院农业生物技术研究所,长春130033 [2]吉林农业大学生命科学学院,长春130118

出　　处：《玉米科学》2015年第5期39-48,共10页Journal of Maize Sciences

基　　金：国家自然科学基金(31100242)

摘　　要：HKT(High-affinity potassium transporter,高亲和性钾转运蛋白)基因所编码的蛋白能起到维持植物体内的Na+/K+平衡的作用。在作物中HKT蛋白主要通过排出叶片中的Na+,从而提高植物耐盐性。对54份玉米自交系材料的Zm HKT1基因及上游序列进行多态性分析。结果表明,在3 784 bp的序列中,共有194个SNP和32个In Del。大部分变异集中在基因上游序列和内含子区,编码区的变异相对较少,说明编码区在进化和人工选择中承受了更大的选择压力。根据序列变异情况可将54份玉米自交系材料划分为15个单倍型,上游序列主要存在3种单倍型,而基因序列存在9种单倍型。遗传重组和连锁不平衡分析发现,全长序列只有3次重组事件发生,上游序列存在着非常强的连锁不平衡。HKT（high-affinity potassium transporter） genes are known to be important in the regulation of Na＋and K＋transportation in higher plants. In crops, the major contribution of the HKT in salt tolerance is the exclusionof Na＋from leaves. In order to illustrate the sequence polymorphism of maize gene Zm HKT1, the Zm HKT1 genes in54 maize inbred lines were sequenced, using the sequence of B73 as the reference. One hundred and ninety-fourSNPs and thirty-two In Dels were identified in the 3 784 bp nucleotide sequences of Zm HKT1. Most of these poly-morphic sites were found to be located in the upstream sequence and introns, forty-two SNPs and three In Dels werein the coding regions, some of which may change the amino acid sequences. The SNPs and In Dels of Zm HKT1 clas-sified all the tested inbred lines into 15 haplotypes. The polymorphic sites in the upstream sequences and gene se-quences clustered the lines into three and nine haplotypes, respectively. Recombination and linkage disequilibriumanalysis indicated that three recombination events had occurred in Zm HKT1 locus and the linkage disequilibrium isstrong in the upstream sequences.

关 键 词：玉米 ZmHKT1基因 单核苷酸多态性(SNP) INDEL 连锁不平衡 单倍型 

分 类 号：S513.035.3[农业科学—作物学]