射频消融术对原发性肝癌患者外周血淋巴细胞亚群的影响  

Changes of Peripheral Blood Lymphocyte Subsets of Patients with Primary Hepatocellular Carcinoma Treated with Radiofrequency Ablation

作　　者：刘江伟[1] 黄建钊[1] 孙倩[1] 芶欣[1] 柳严[1] 赵鹏伟[1] 张佳伟[1] 

机构地区：[1]贵州省人民医院肝胆外科,贵州贵阳550002

出　　处：《中国普外基础与临床杂志》2015年第10期1171-1174,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：贵州省科技厅联合基金资助(项目编号:黔科合LS字[2011]004号);贵州省卫生厅基金资助(项目编号:gzwkj2010-1-013)~~

摘　　要：目的观察射频消融(radiofrequency ablation,RFA)治疗前后原发性肝细胞癌患者淋巴细胞亚群的变化。方法收集笔者所在贵州省人民医院2011年7月至2014年12月期间经临床及肝脏穿刺组织病理学确诊为原发性肝细胞癌且自愿接受经皮肝癌射频消融治疗的70例住院患者资料。根据肝内病灶数量,将其分为单病灶组(n=41)和多病灶组(n=29),分析其RFA治疗前后免疫指标的变化。结果 1 70例患者RFA治疗后7 d及14 d外周血CD3+/CD19-、CD3+/CD4+和CD4+/CD8+T淋巴细胞比值以及NK细胞较RFA治疗前1 d明显升高,其差异均有统计学意义(P<0.05);而RFA治疗后7 d及14 d外周血CD3+/CD8+T淋巴细胞较RFA治疗前1 d有所降低,但差异无统计学意义(P>0.05)。2单病灶组和多病灶组患者RFA治疗前后外周血淋巴细胞亚群的变化趋势与上述改变类似。3多病灶组RFA治疗前后外周血CD3+/CD19-、CD3+/CD4+及CD4+/CD8+T淋巴细胞比值和NK细胞均低于单病灶组,而CD3+/CD8+T淋巴细胞比值则高于单病灶组,但差异均无统计学意义(P>0.05)。结论 RFA治疗小肝癌不仅能毁灭癌灶,还能使机体免疫功能显著改善,抗肿瘤效应增强。Objective To observe the changes of peripheral blood lymphocyte subsets of patients with primary hepatocellular carcinoma （PHCC） treated with radiofrequency ablation （RFA）. Methods The data of 70 cases of hospitalized patients with PHCC that voluntary accepted RFA diagnosed by clinical and pathological in our hospital between July 2011 and December 2014 were collected. According to the numbers of HCC lesions, 70 cases were divided into single focus group （n=41） and multifocal group （n=29）. The changes of their immune parameters before and after RFA were analyzed. Results （1） The ratioes of peripheral blood CD3＋/CD19-, CD3＋/CD4＋, CD4＋/CD8＋, and NK cells on 7 days and 14 days after RFA treatment of 70 cases were significantly higher than those on 1 day before RFA treatment （P〈0.05）. The ratio of CD3＋/CD8＋ T cells reduced from 1 day before RFA treatment to 14 days after RFA treatment, but the difference was no statistically significant （P〉0.05）. （2） The changing trend of peripheral blood lymphocyte subsets before and after RFA treatment in single focus group and multifocal group were similar to the above. （3） Compared with single focus group, the ratioes of peripheral blood CD3＋/CD 19-, CD3＋/CD4＋, CD4＋/CD8＋, and NK cells before and after RFA treatment in muhifocal group were lower, and the ratio of CD3＋/CD8＋ T cells was higher, but the difference were not statistically significant （P〉0.05）. Conclusion RFA can not only destroy small PHCC loci, but also to significantly improve immune function and enhance the anti-tumor effect.

关 键 词：原发性肝细胞癌 射频消融 淋巴细胞亚群 

分 类 号：R735.7[医药卫生—肿瘤]