人血清白蛋白对大鼠蛛网膜下腔出血后血脑屏障及神经功能的影响  

Effects of human albumin on the blood-brain barrier and behavior changes in subarachnoid hemorrhage rats

作　　者：刘莹[1] 王刘敏 谢怡[2] 李鹏[1] 叶瑞东[2] 刘娟芳[3] 

机构地区：[1]第四军医大学学员一旅,西安710032 [2]南京军区南京总医院神经内科,南京210002 [3]第四军医大学航空航天医学院航空航天临床医学教研室,西安710032

出　　处：《神经解剖学杂志》2015年第5期551-556,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81300926;31300900;81471182;81200892;81400332)

摘　　要：目的:探讨人血清白蛋白对于蛛网膜下腔出血(SAH)后血脑屏障及行为学的影响。方法:将雄性大鼠随机分为假手术组、生理盐水组、低剂量人血清白蛋白治疗组(0.63g/kg)和高剂量白蛋白治疗组(1.25g/kg)。采用刺破法制造大鼠SAH模型,利用改良Garcia评分系统及平衡木实验评估SAH后24 h及14 d行为学的变化,利用伊文思蓝外渗和Ig G染色评估血脑屏障破坏情况,以蛋白免疫印迹法观察基质金属蛋白酶-2(MMP-2)及基质金属蛋白酶-9(MMP-9)的表达情况。结果:与生理盐水组相比较,低剂量和高剂量白蛋白治疗组大鼠SAH后24 h及14 d神经功能缺损评分及平衡木评分均明显降低(P<0.05),伊文思蓝渗出均减少(P<0.05),血管周围lg G渗出均减少,患侧组织中MMP-2及MMP-9的表达均明显减少(P<0.05),且低剂量和高剂量白蛋白治疗效果无明显差异(P>0.05%)。结论:SAH后给予白蛋白治疗,可以降低MMP-2及MMP-9的表达,减轻出血继发的血脑屏障的破坏,改善神经功能缺损。Objective： To investigate the effect of albumin therapy on blood-brain barrier and behavior. Methods：Male rats were divided into four groups： sham operation group, saline group, low dose of albumin therapy group （0.63 g/kg） and high dose of albumin therapy group（ 1.25 g/kg）. SAH was induced by the endovascular perforation rat model. Neu- rological outcomes were assessed by a blinded observer at 24 h and 14 d post SAH using the modified garcia score and beam balance test. The blood-brain barrier disruption was assessed by evans blue content analysis and IgG staining. Western blot was used to determine the expression of MMP-2 and MMP-9. Results：Compared with saline group, the neu- rological outcomes of SAH rats were improved upon both low and high dose of albumin therapy at 24 h and 14 d post SAH. Both the content of Evans blue and the IgG staining were decreased. Western bolt showed the reduced expression of MMP-2 and MMP-9 in low and high dose of albumin group. There are not significant difference between low dose of albu- min group and high dose of albumin group. Conclusion： Albumin therapy can decrease the expression of MMP-2 and MMP-9, reduce BBB disruption, and thereby improve neurological outcomes in subarachnoid hemorrhage rats.

关 键 词：蛛网膜下腔出血 人血清白蛋白 血脑屏障 大鼠 

分 类 号：R743.35[医药卫生—神经病学与精神病学]