重庆黄连种植土壤中酚酸类物质累积与消减规律的研究  

Study on Accumulation and Decrease Law of Phenolic Acids in Soil of Cultivation Base of Coptis chinensis

作　　者：张丹[1,2] 钟国跃[3] 曹纬国[2] 瞿显友[4,5] 廖光平[4,5] 

机构地区：[1]成都中医药大学药学院,成都611137 [2]重庆医科大学中医药学院,重庆400016 [3]江西中医药大学中药资源与民族药研究中心,南昌330004 [4]重庆市中药资源学重点实验室,重庆400065 [5]中国中医科学院中药资源中心重庆分中心,重庆400065

出　　处：《世界科学技术-中医药现代化》2015年第7期1419-1424,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：中医药管理局国家公益性行业科研专项课题(201207002-04):重庆市代表性区域特色中药资源保护与利用;负责人:钟国跃;重庆市科委科技平台与基地建设项目(cstc2012pt-sy0006):中药资源学重庆重点实验室;负责人:瞿显友

摘　　要：目的:建立黄连种植土壤中酚酸类物质的分析方法,探索其累积与消减规律。方法:采用Fe Cl3-K3[Fe(CN)6]比色法测定黄连种植土壤中总酚酸的含量,采用HPLC法测定黄连种植基地土壤中阿魏酸的含量。结果:测得黄连种植基地土壤中总酚酸含量约0.545-2.026 mg·100g-1在范围内,阿魏酸含量在0.139-0.652μg·100g-1范围内。结论:黄连种植土壤中酚酸含量变化规律明显,栽培过程中随黄连生长年限的增加,连地土壤中总酚酸与阿魏酸的含量均表现为增加,采收黄连后的休地过程中,土壤中总酚酸与阿魏酸的含量均呈现递减趋势,该变化趋势可为黄连连作障碍研究机制与解决方法研究提供了参考。This study was aimed to establish a method to determine the content of phenolic acids in the soil of cultivation base ofCoptis chinensis, in order to study the accumulation and decrease law of phenolic acids. The content of total phenolic acid was determined by ferric chloride-ferricyanatum calcium colorimetric method. Thecontent of ferulic acid in Coptis chinensis was determined by HPLC. The results showed that the contents of total phenolic acid and ferulic acid in the soil of cultivation base of Coptis chinensis were in the range from 0.545-0.026 mg·g-1 and 0.139 to 0.652 μg·g-1, respectively. It was concluded that the variation of phenolic acids in the soil of cultivation base ofCoptis chinensis was obvious. With the increase of growth age of Coptis chinensis, the contents of total phenolic acid and ferulic acid in the soil of cultivation base of Coptis chinensis were increased in the cultivation period. With the increase of fallow age, the contents of total phenolic acid and ferulic acid in the soil of cultivation base ofCoptis chinensis showed decrease tendency in the fallow period of Coptis chinensis. The variation tendency of phenolic acids contents in the soil of cultivation base ofCoptis chinensis can be referred to in the study of the continuous cropping obstacle of Coptis chinensis.

关 键 词：黄连 土壤 总酚酸 阿魏酸 

分 类 号：R284[医药卫生—中药学]