甜茶多酚对自发性高血压大鼠的影响  

Effect of Polyphenols from Rubus suavissirnu S. Lee on Spontaneous Hypertensive Rats

作　　者：蒋荣华[1] 候小利[2] 王硕[2] 周小雷[2] 龚小妹[2] 缪剑华[2] 

机构地区：[1]广西医科大学药学院,南宁530021 [2]广西药用植物研究所西南濒危药材资源开发国家工程实验室,南宁530023

出　　处：《世界科学技术-中医药现代化》2015年第7期1479-1485,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：广西中医药管理局科技攻关项目(GZYZ1217):瑶药复方甜茶降脂合剂的开发研究;负责人:周小雷;缪剑华

摘　　要：目的:研究广西甜茶多酚(RSLP)对自发性高血压大鼠(SHR)的影响,并探讨其降血压的作用机制。方法:采用水提法制备RSLP浸膏,并过大孔树脂进行待试样品甜茶多酚的制备,无创血压分析系统用于检测血压。选择SHR模型研究甜茶多酚降血压的作用,将正常Wistar大鼠16只,随机分为正常组、甜茶多酚正常高剂量组;SHR大鼠40只,随机分为模型组、阳性药卡托普利组、甜茶多酚低、中、高剂量组。连续给药8周及停药2周后,采用无创尾动脉血压分析系统,观察甜茶多酚对大鼠收缩压(SBP)、舒张压(DBP)、心率(HR)、体质量及脏器指数的影响。测定血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮(NO)、一氧化氮合酶(NOS)、心房钠尿肽(ANP)含量。结果:SHR血压明显高于正常组,可用于RSLP降血压实验研究。1 RSLP各组能够明显降低SHR大鼠的SBP和DBP(P<0.05),但是对心率HR无影响(P>0.05);2广西甜茶多酚RSLP能升高各给药组血清中GSH-Px、SOD含量(P<0.05),降低MDA活性(P<0.05);3 RSLP能降低各给药组血清中NO、NOS、ANP含量(P<0.05)。结论:RSLP能明显降低SHR的SBP和DBP,而对HR无明显影响。能够提高血清中GSH-Px、SOD、NO、NOS活性,降低MDA、ANP含量,对左心室肥厚也有一定的抑制作用。This article was aimed to study the effect of polyphenols fromRubus suavissirnusS. Lee （RSLP） on spontaneous hypertensive rats （SHR） and to explore its mechanism of anti-hypertensive. The water extraction of RSLP was prepared. And the polyphenols was extracted with macroporous resin. The non-invasive blood pressure analysis system was used to detect the blood pressure. SHR model was selected to study the anti-hypertensive effect. The 16 normal Wistar rats were randomly divided into the control group and the normal RSLP high-dose group （RSLP-NH）. The 40 SHR were randomly divided into the model group, Captopril group, RSLP-L group, RSLP-M group and RSLP-H group. SBP, DBP, HR, body weight and organ index were observed after the drug administration for 8 weeks and drug withdrawal for 2 weeks. The contents of SOD, MDA, GSH-Px, NO, NOS and ANP in serum were measured. The results showed that the blood pressure of SHR was significantly higher than that of the control group, which can be used for anti-hypertensive studies. Each RSLP group can obviously reduce the SBP and DBP of SHR （P 〈 0.05）, but it had no effect on HR （P 〉 0.05）. RSLP can elevate GSH-Px, SOD levels and reduce the activity of MDA （P 〈 0.05）. RSLP can reduce NO, NOS and ANP contents in serum （P 〈 0.05）. It was concluded that RSLP can significantly reduce the SBP and DBP of SHR, but it had no significant effect on HR. It can increase the activity of GSH-Px, SOD, NO, NOS levels, and reduce the contents of MDA, ANP in serum. It had certain inhibitory effect on the left ventricular hypertrophy.

关 键 词：广西甜茶 多酚 自发性高血压大鼠 降血压 

分 类 号：R285.5[医药卫生—中药学]