食管、胃双原发癌Her-2、人乳头瘤病毒表达与临床病理特征的关系  

The relationship between expression of Her-2 and human papillomavirus and clinicopathological features in esophageal and stomach multiple primary cancers

作　　者：张夏林[1] 李建民[2] 孙瑞芳[2] 白玮[2] 

机构地区：[1]山西医科大学基础医学院,太原030001 [2]山西医科大学附属肿瘤医院病理科

出　　处：《肿瘤研究与临床》2015年第9期597-601,共5页Cancer Research and Clinic

摘　　要：目的：分析上消化道双原发癌中Her-2蛋白表达和人乳头瘤病毒（HPV）感染情况与临床病理因素的关系，探讨同一系统中不同组织类型的恶性肿瘤在发生发展过程中的联系。方法39例患者均为原发性食管鳞状细胞癌、胃／贲门腺癌。采用免疫组织化学法检测双原发癌标本中Her-2蛋白的表达，采用原位杂交技术检测HPV-DNA表达情况。结果 Her-2蛋白在食管鳞状细胞癌的过表达与鳞状细胞癌分化程度、浸润深度、淋巴结转移及患者的年龄、性别均无关（P＞0.05），Her-2在胃腺癌的过表达与腺癌浸润深度、淋巴结转移相关（P＜0.05），与患者的年龄、性别、分化程度无关（P＞0.05）。食管鳞状细胞癌和胃腺癌患者HPV感染与二者的肿瘤分化程度、浸润深度、淋巴结转移、患者的年龄、性别均无关（P＞0.05）。在上消化道双原发癌肿瘤组织中，Her-2蛋白的过表达呈一致性（κ＝0.75，P＜0.05）。同时， HPV-DNA的表达也呈一致性（κ＝0.80，P＜0.05）。结论 Her-2蛋白和HPV在上消化道双原发癌中均存在一定的一致性表达，可能是食管鳞状细胞癌和胃腺癌的共同相关致病危险因素。Objective To analyze the relationship between expression of Her-2 protein and the infection of human papillomavirus （HPV） and clinicopathological features in upper gastrointestinal multiple primary cancers, and to explore the relationship between the different histological malignancies in a single-system. Methods 39 patients were primary esophageal squamous cell carcinoma and gastric/cardia adenocarcinoma. By using immunohistochemistry （IHC） methods, the expression of Her-2 protein in 39 cases of multiple primary cancers specimens were examined, and by using insitu hybridization （ISH） technology, the infection of HPV in the same ones were detected. Results The over-expression of Her-2 protein in esophageal squamous cell carcinoma was not significantly relative to the degree of differentiation, the depth of penetration, the lymph node metastasis and the patientsˊage and gender （P〈 0.05）. The over-expression of Her-2 protein in gastric adenocarcinoma was closely correlated to the invasion depth and lymph node metastasis （P〉0.05）, no obvious correlation with the factors of patientsˊage and gender and the degree of differentiation （P 〈 0.05）. The infection of HPV in esophageal squamous cell carcinoma and gastric adenocarcinoma was not significantly relative to the degree of differentiation, the depth of penetration,the lymph node metastasis, and the age and gender of the patients （P〈 0.05）. In the upper gastrointestinal multiple primary cancers, the Her-2 protein over-expression had the consistency （κ= 0.56, P〉 0.05）. Meanwhile, the HPV-DNA expression also had the consistency （κ=0.80, P〉0.05）. Conclusion Both Her-2 protein and HPV expression show the consistency in upper gastrointestinal multiple primary cancers,which suggests that Her-2 protein and HPV may be the common oncogenic factors for both esophageal squamous cell carcinoma and gastric adenocarcinoma.

关 键 词：食管肿瘤 癌 鳞状细胞 胃肿瘤 腺癌 HER-2蛋白 人乳头瘤病毒 

分 类 号：R735[医药卫生—肿瘤]