姜黄素诱导黑素瘤细胞凋亡的研究  

Curcumin results melanoma cells apoptosis

作　　者：王红梅[1] 韩正祥[1] 杜秀平[1] 李滔涛[1] 蒋冠[2] 

机构地区：[1]徐州医学院附属医院肿瘤科,江苏徐州221000 [2]徐州医学院附属医院皮肤科,江苏徐州221000

出　　处：《临床与病理杂志》2015年第9期1653-1657,共5页Journal of Clinical and Pathological Research

摘　　要：目的:研究姜黄素诱导黑素瘤细胞A375、M14凋亡的机制。方法:将对数生长的黑素瘤细胞A375、M14分别用姜黄素干预;显微镜下观察黑素瘤细胞株A375和M14的凋亡状态;应用Western Blot方法检测凋亡相关蛋白Bcl-2、Ba x、Caspase-3、γ-H2A X、NF-κB的变化;应用流式细胞仪检测细胞凋亡率。结果:实验发现姜黄素干预黑素瘤细胞后可见细胞发生凋亡;在姜黄素诱导黑素瘤细胞凋亡过程中,凋亡相关蛋白Bax和γ-H2AX表达水平较对照组升高,而Bcl-2、NF-κB表达水平较较对照组下降,有活性的Caspase-3生成;随着姜黄素的浓度增加,黑色素瘤细胞早期凋亡的比例增加。结论:姜黄素诱导黑素瘤细胞生长凋亡的机制可能与其对凋亡相关蛋白Bcl-2、Bax、Caspase-3、γ-H2AX、NF-κB的表达调控有关。Objective：To observe the effects of curcumin on the apoptosis of two melanoma cell lines A375 and M14. Methods：Cell apoptosis in human melanoma cell lines A375 and M14 treated with curcumin was measured by morphological method. Furthermore four apoptosis related proteins：Bcl-2, Bax, Caspase-3,γ-H2AX and NF-κB were determined by Western Blotting analysis. Apoptosis rate were detected through FCM （lfow cytometry）. Results：hTe data showed that curcumin can induce tumor cell apoptosis. Western blotting analysis conifrmed that curcumin could express increasing proteins Bax andγ-H2AX, down-regulating the proteins Bcl-2, and NF-κB, as well as, activation of Caspase-3. It showed that with the increase of the concentration of curcumin, melanoma cell early apoptosis ratio increased. Conclusion：hTis study showed that curcumin exhibited a remarkably increased apoptosis-inducing effects in melanoma cells.

关 键 词：姜黄素 黑素瘤 凋亡 

分 类 号：R739.5[医药卫生—肿瘤]