新生小鼠缺氧缺血性脑病脑组织caspase-3表达的变化  

Increased expression of caspase-3 in ipsilateral neonatal hypoxic-ischemia encephalopathy model in mice

作　　者：肖爱娇[1] 康明非[2] 汪建民[3] 罗小泉[4] 

机构地区：[1]江西中医药大学生理学学科组,330004 [2]江西中医药大学针灸康复科,330004 [3]江西中医药大学解剖学学科组,330004 [4]江西中医药大学实验动物科技中心,330004

出　　处：《天津医药》2015年第10期1116-1118,I0002,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(81060305);江西中医药大学课题(2014ZR018);江西省卫生计生委中医药科研计划项目(2014Z003);江西省自然科学基金资助项目(20151BAB205068)

摘　　要：目的观察半胱氨酸天冬氨酸蛋白酶(caspase)-3在新生儿缺氧缺血性脑病(NHIE)小鼠模型脑组织中表达的变化。方法选取7 d CD1新生小鼠30只,按随机数字表法分为假手术组(9只)和NHIE模型组(21只),后者制备NHIE动物模型。用TTC染色法检查2组的脑组织梗死面积;DAPI染色观察脑组织病理变化;原位末端标记技术检测脑组织细胞凋亡;荧光免疫组化法检测脑组织中caspase-3表达水平。结果假手术组小鼠脑组织未见梗死灶,脑组织细胞排列致密整齐,TUNEL阳性细胞数[(18.57±4.98)个]和caspase-3阳性细胞数[(9.17±2.14)个]明显低于NHIE模型组的TUNEL阳性细胞数[(209.57±41.27)个]和caspase-3阳性细胞数[(63.33±16.22)个];与假手术组相比,NHIE模型组小鼠的右侧半球可见梗死灶,脑组织细胞大量坏死脱落、周围组织间隙变大。结论 NHIE模型鼠出现的脑组织损伤可能与caspase-3表达增加、脑组织细胞凋亡增加有关。Objective To observe apoptotic cells and caspase-3-positive cells in ipsilateral neonatal hypoxic-isch-emia encephalopathy （NHIE） model in mice. Methods CD1 mice of age 7 days （n=30） were randomly divided into two groups： sham group （n=9） and model group （n=21）. NHIE model was induced by right common carotid artery ligation fol-lowed by 8%oxygen hypoxia for 100 min. TTC staining was used to determine area of brain infarction. DAPI staining was used to detect pathological change in brains. TUNEL assay was used to detect apoptotic cells and fluorescence immunohisto-chemistry was used to detect caspase-3 expression in the ipsilateral brain. Results No infarct was detected in sham group. Cells were densely and orderly arranged in brain. TUNEL-positive cells （18.57±4.98） and caspase-3-positive cells （9.17± 2.14） in the ipsilateral brain were both less than those in the ipsilateral brain of mice in model group （209.57±41.27） and （63.33±16.22） respectively. Mice in model group presented infarct in the right hemisphere with more dead cells and wider in-terstitial space compared with sham group. Conclusion Brain injury in NHIE model might be related to the increasing cas-pase-3 expression thus leads to apoptosis.

关 键 词：缺氧缺血 脑梗死 细胞凋亡 半胱氨酸天冬氨酸蛋白酶3 小鼠 缺氧缺血性脑病 

分 类 号：R742[医药卫生—神经病学与精神病学]