犬动脉瘤动物模型的血流动力学分析  

Haemodynamic analysis in canine aneurysm model

作　　者：李浩[1,2] 马勃[3] 李志强[2] 王启弘[4] 

机构地区：[1]南方医科大学,广州510515 [2]南方医科大学附属奉贤医院、上海交通大学附属第六人民医院南院神经外科 [3]安徽理工大学病原免疫学教研室 [4]上海交通大学医学院附属瑞金医院神经外科

出　　处：《天津医药》2015年第10期1133-1136,I0005,共5页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(30872676)

摘　　要：目的：探讨建立稳定、理想、快捷的颅内动脉瘤模型的方法，为动脉瘤的相关研究提供可靠的动脉瘤模型。方法在改良的外科显微技术条件下制作侧壁型、分叉部、末端型和真性合并假性囊状动脉瘤，每种类型各7只。术后2周行彩色多普勒超声仪、磁共振成像（MRI）、磁共振血管成像（MRA）、数字减影动脉血管造影（IADSA）检查，进行动脉瘤模型的计算机流体力学（CFD）模拟分析。采用体外细胞培养的方法，对动脉瘤壁的组织生物力学特性进行分析，以微弹簧圈（MC）紧密填塞动脉瘤并再次进行上述检查。结果所有模型均获得成功，MC瘤腔栓塞能取得良好效果。培养证实血管内皮细胞（VEC）的体外增殖情况良好，并成功将VEC贴附于高分子可降解材料表面，进而生长成VEC层结构。对栓塞前后的动脉瘤模型进行了血流动力学分析，获得了相关的血流动力学参数，动脉瘤内的血流速度为2～7 cm/s，动脉瘤载瘤动脉内的血流速度为8～26 cm/s。结论建立了稳定、理想、快捷的颅内动脉瘤模型，可为动脉瘤的相关研究提供可靠的动脉瘤模型。Objective To explore a way to establish a stable, ideal and handy intracranial aneurysm model for related studies. Methods Modified microsurgery technology was applied to establish sidewall, bifurcation, terminal and merged types of aneurysm （n=7 in each type）. Two weeks after operation, color Doppler ultrasound, magnetic resonance imaging （MRI）, magnetic resonance angiography （MRA） and intra-arterial digital subtraction angiography （IADSA） were performed and computational fluid dynamics （CFD） was used for simulation analysis of aneurysm models. Biomechanical properties of aneurysm wall tissues was analyzed by cell culture, then the above examinations were performed again after the aneurysm was embolized by micro coil （MC）. Results All models were successfully established, and the MC embolization of aneurysm cavity achieved good efficacy. Vascular endothelial cells （VECs） proliferate well in vitro, and the VECs adhered to the sur？face of biodegradable polymeric materials successfully and grow into VEC layer structure. Hemodynamics analysis was per？formed in aneurysms model before and after embolization and relevant hemodynamic parameters was obtained. , Blood flow velocity are 2-7 cm/s in aneurysm and 8-26 cm/s in its parental artery. Conclusion A stable, ideal and handy intracranial aneurysm model was established and are essential in aneurysm related research.

关 键 词：血流动力学 颅内动脉瘤 动物模型 

分 类 号：R743.4[医药卫生—神经病学与精神病学]