MTA3在肺癌细胞中调控细胞凋亡机制的研究  

Regulation Mechanism of MTA3 in the Apoptosis of NSCLC Cells

作　　者：李海英[1] 王庆苓[1] 张琳[1] 包海军[1] 张恒[1] 

机构地区：[1]徐州医学院基础医学院病理教研室,徐州221000

出　　处：《中国肺癌杂志》2015年第10期610-615,共6页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金项目(No.81101493;No.81302612);徐州医学院人才项目启动基金(No.D2014003)资助~~

摘　　要：背景与目的肿瘤转移基因(metastasis associated gene,MTA)是一个肿瘤候选基因家族,主要包括MTA1、MTA2、MTA3,已有的研究证实在不同肿瘤中MTA3发挥着相反的作用,本研究旨在探讨MTA3在肺癌细胞中调控细胞凋亡方面的影响。方法应用Western blot方法和Real-time PCR方法检测肺癌细胞系A549和H157中MTA3的转染效率,流式细胞仪方法检测上调/下调MTA3后肺癌细胞凋亡情况,Western blot方法检测下调MTA3后凋亡相关基因的表达。结果在肺癌细胞系A549和H157中干扰MTA3后则促进细胞凋亡,同时引起凋亡相关蛋白Bax、ClevedCaspase-3、p-PARP表达上调及Bcl-2表达下调。结论 MTA3在肺癌细胞系A549和H157细胞中通过抑制凋亡相关基因的表达抑制细胞凋亡。Background and objective Metastasis-associated protein 3 （MTA3） was originally found as a member of a small protein family （including MTA1, MTA2 and MTA3）, and it has been proven that MTA3 had different roles in dif- ferent types of human cancers. The aim of this study is to explore the function of MTA3 to regulate the cell apoptosis in lung cancer. Methods Western blot and Real-time PCRwere used to detect the expression level of MTA3 after transfection in non- small cell lung cancer （NSCLC） ceIIs A549 and H157. Apoptosis analysis was used to detect the change of cell apoptosis with upregulated/downregulated of MTA3, and Western blot was used to detect the the expression of the protein related with apoptosis, while dowrtregulate the expression of MTA3 in NSCLC ceils A549 and H157. Results Downregulated of endogenous MTA3 could promote apoptosis in NSCLC cells, meanwhile, siMTA3 could upregulate the protein ofBAX, Cleved-Caspase-3, p-PARP, and dowmregulate the protein of Bcl-2. Conclusion The data we present here indicate that MTA3 suppress apoptosis ofA549 an H157 cells by inhibiting BAX, PARP expression.

关 键 词：肺肿瘤 肿瘤转移相关基因3 细胞凋亡 A549 H157 

分 类 号：R734.2[医药卫生—肿瘤]