肺鳞癌EGFR与KRAS基因突变状态分析  

Detection and Analysis of EGFR and KRAS Mutations in the Patients with Lung Squamous Cell Carcinomas

作　　者：张卉[1,2] 杨新杰[1,2] 秦娜[1,2] 李曦[1,2] 杨惠夷 农靖颖[4] 吕嘉林[1,2] 吴羽华[1,2] 张权[1,2] 张新勇[1,2] 王敬慧[1,2] 苏丹[1,5] 张树才[1,2] 

机构地区：[1]首都医科大学附属北京胸科医院 [2]北京市结核病胸部肿瘤研究所肿瘤内科,北京101149 [3]益善医学检验所,广州510663 [4]首都医科大学附属北京胸科医院/北京市结核病胸部肿瘤研究所肿瘤内科,北京101149 [5]北京市结核病胸部肿瘤研究所病理科,北京101149

出　　处：《中国肺癌杂志》2015年第10期621-625,共5页Chinese Journal of Lung Cancer

基　　金：首都临床特色研究项目(No.Z121107001012081)资助~~

摘　　要：背景与目的表皮生长因子受体(epidermal growth factor receptor,EGFR)突变和KRAS基因突变是非小细胞肺癌(non-small cell lung cancer,NSCLC)靶向治疗的重要分子标志,但关于肺鳞癌中EGFR和KRAS基因突变情况的报道甚少。本研究旨在分析肺鳞癌EGFR和KRAS基因突变与临床特征的关系。方法收集初治肺鳞癌患者139例,有可供检测的肿瘤组织标本。利用突变富集液相芯片法进行EGFR和KRAS基因突变检测。结果 139例肺鳞癌中,EGFR基因突变25例(18%),KRAS基因突变7例(5%),EGFR和KRAS基因同时发生突变1例(0.7%)。女性和不吸烟患者EGFR基因突变率高于男性和吸烟患者(33.3%vs 16.5%,29.6%vs 16.1%),但差异均无统计学意义(P>0.05,P>0.05);不同年龄、分期及病理取材标本之间差异均无统计学意义(P>0.05)。男性患者KRAS基因突变率高于女性患者(5.5%vs 0%),但差异无统计学意义(P>0.05);在不同年龄、分期、病理取材标本及是否吸烟各亚组分析中KRAS基因突变差异无统计学意义(P>0.05)。结论肺鳞癌患者EGFR和KRAS基因突变发生率均较低,且都与临床特征无明显相关。肺鳞癌患者使用酪氨酸激酶抑制剂(tyrosine kinase inhibitors,TKIs)靶向治疗药物之前,也应检测EGFR基因和KRAS基因的突变情况。Background and objective Activating mutations in epidermal growth factor receptor （EGFK） and KRAS are important markers in non-small cell lung cancer. However, EGFR and KRAS gene mutations in lung squamous cell carcinoma are rarely reported. The aim of this study was to analyze EGFR and KRAS gene mutation rate and their relationship with clinical features in patients with lung squamous cell carcinomas. Methods A total of 139 patients undergoing treatment for naive lung squamous cell carcinomas with tumor tissue samples available for testing were recruited. EGFR and KRAS mutation statuses of the tumor samples were detected using a mutant enriched liquid chip. Results Of the 139 cases of lung squamous cell carcinoma, EGFR mutations were detected in 25 cases （18%）, KRAS mutations were detected in 7 cases （5%）, and the pres- ence of both EGFR and KRAS mutations was detected in 1 case （0.7%）. EGFR mutations occurred more often in females than in males （33.3% vs 16.5%） and in patients that never smoked than in those who smoke （29.6% vs 16.1%）. However, the difference did not reach statistical significance （P〉0.05）. No significant differences were observed in age, stage, and different biopsy type. KRAS mutations occurred more often in males than in females （5.5% vs 0%）, but the difference did not reach statistical significance （P〉0.05）. No significant differences were observed in age, stage, different biopsy type, and smoking status （P〉0.05）. Condusion EGFR and KRAS mutations were low in lung squamous cell carcinomas, and had no significant correlation with clinical features. Before using tyrosine kinase inhibitor targeted therapy, EGFR and KRAS mutations should be detected in pa- tients with lung squamous cell carcinomas.

关 键 词：肺肿瘤 表皮生长因子受体 KRAS基因 突变 鳞癌 

分 类 号：R734.2[医药卫生—肿瘤]