通窍活血汤对脑梗死急性期大鼠胃动素及血管活性肠肽的调节作用  

The regulatory effect of Tongqiao Huoxue decoction on motilin and vasoactive intestinal peptide in rats with cerebral infarction at acute stage

作　　者：唐明 安朋朋 任志珍 唐晓晓 李红 杨秀丽 

机构地区：[1]青岛市海慈医疗集团,山东青岛266033 [2]青岛市市南区社区卫生服务中心,山东青岛266000 [3]青岛开发区第一人民医院,山东青岛266555

出　　处：《中国中西医结合急救杂志》2015年第5期462-466,共5页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：山东省中医药科技发展计划项目（2009-203）

摘　　要：目的 研究通窍活血汤对脑梗死急性期大鼠胃动素（MTL）及血管活性肠肽（VIP）的影响,以及对胃黏膜的保护作用.方法 将96只Wistar大鼠按随机数字表法分为正常对照组、假手术组、脑梗死模型组、西咪替丁组、通窍活血汤常规剂量组和通窍活血汤低剂量组,每组16只.采用线栓法制备大鼠大脑中动脉梗死模型,制模后脑梗死模型组、假手术组用2 mL生理盐水灌胃;通窍活血汤常规剂量组用1.3 g/mL通窍活血汤2 mL灌胃;通窍活血汤低剂量组用1.3 g/mL通窍活血汤1 mL加1 mL生理盐水灌胃;西咪替丁组用0.1 g/kg西咪替灌胃;正常对照组不进行任何处理.于给药后4 d、7 d取血测定血浆MTL、VIP含量;分离胃组织,电镜观察下观察组织结构改变.结果 正常对照组和假手术组MTL、VIP含量比较差异均无统计学意义（均P〉0.05）; 给药后4 d、7 d脑梗死模型组血浆MTL、VIP含量均较正常对照组和假手术组明显增多〔4 d：MTL（ng/L）：189.51±13.48比117.01±11.38、117.67±12.73,VIP（ng/L）：94.86±4.14比60.98±5.42、62.55±6.60,均P〈0.05;7 d：MTL（ng/L）：183.05±14.49比119.79±10.64、120.27±11.48,VIP（ng/L）：91.92±3.77比59.63±4.29、58.10±4.99,均P〈0.05〕;西咪替丁组、通窍活血汤常规剂量组和通窍活血汤低剂量组均较脑梗死模型组明显降低,且以通窍活血汤常规剂量组给药后7 d血浆MTL、VIP含量降低程度较西咪替丁组和通窍活血汤低剂量组更显著〔MTL（ng/L）：138.72±8.02比152.16±12.66、156.60±11.87,均P〈0.05;VIP（ng/L）：68.18±3.41比81.12±3.98、78.89±2.13,均P〈0.05〕.电镜下可见：与正常对照组及假手术组比较,脑梗死模型组大鼠胃黏膜结构出现明显组织破坏;通窍活血汤常规组、通窍活血汤低剂量组和西咪替丁组上述组织破坏均减轻,且通窍活血常规组的疗效更优.结论 脑梗死急性期胃黏膜出现明显损害;通窍活血汤不但能有效调节�Objective To observe the effect of Tongqiao Huoxue decoction on the changes of motilin （MTL） and vasoactive intestinal peptide （VIP） and its protective effect on gastric mucosa in rats with cerebral infarction at acute stage.Methods According to random number table, 96 Wistar rats were divided into six groups： normal control, sham operation, cerebral infarction model, cimetidine, Tongqiao Huoxue decoction routine dose and low dose groups, eachn = 16. Cerebral infarction model group was established by using a suture to ligate the middle cerebral artery, resulting in its occlusion. The rats of cerebral infarction model group and sham operation group were lavaged with 2 mL of normal saline. Tongqiao Huoxue decoction routine dose group was lavaged with 2 mL of Tongqiao Huoxue decoction （1.3 g/mL）, while Tongqiao Huoxue decoction low dose group was lavaged with 1 mL of Tongqiao Huoxue decoction （1.3 g/mL） plus 1 mL of normal saline. Cimetidine group was lavaged with cimetidine （0.1 g/kg）, and nothing was done for the normal control group. On the 4th and 7th day after the delivery of treatment, inferior vena venous blood samples were collected to detect the plasma MTL and VIP concentrations. Then the gastric tissues were obtained and examined under an electron microscope.Results There were no statistically significant differences between the normal control group and sham operation group in plasma levels of MTL and VIP （bothP 〉 0.05）. The plasma levels of MTL and VIP at 4 days and 7 days after the delivery of treatment in cerebral infarction model group were higher than those in the normal control group and sham operation group [4 days MTL （ng/L）： 189.51±13.48 vs. 117.01±11.38, 117.67±12.73, VIP （ng/L）： 94.86±4.14 vs. 60.98±5.42, 62.55±6.60, bothP 〈 0.05; 7 days： MTL （ng/L）： 183.05±14.49 vs. 119.79±10.64, 120.27±11.48, VIP （ng/L）： 91.92±3.77 vs. 59.63±4.29, 58.10±4.99, both P 〈 0.05]. Plasma levels of MTL and VIP in cimetidine group, Tongqiao Huoxue d

关 键 词：通窍活血汤 脑梗死急性期 胃动素 血管活性肠肽 电镜 

分 类 号：R-332[医药卫生]