HPLC法测定蒙药那如三味丸中没食子酸和胡椒碱的含量  

Simultaneous Determination of Gallic Acid and Piperine in Mongolian Medicine Naru Sanwei Pills by HPLC

作　　者：李立 

机构地区：[1]南宁市食品药品检验所,南宁530001

出　　处：《中国药师》2015年第10期1830-1832,共3页China Pharmacist

摘　　要：目的：建立高效液相色谱法同时测定蒙药那如三味丸中没食子酸和胡椒碱含量的方法。方法：采用Ultimate XB-C18（250 mm×4.6 mm,5μm）为色谱柱;以甲醇-0.1%磷酸为流动相,梯度洗脱（0-20 min,流动相比例15∶85→80∶20;20-30min,流动相比例80∶20→15∶85）,流速为1.0 ml·min-1,检测波长为285 nm,柱温：30℃,进样量：10μl。结果：在此色谱条件下,没食子酸和胡椒碱达到基线分离,且线性良好,线性范围分别为0.878-4.392μg（r=1.000 0）和0.262-1.308μg（r=1.000 0）;平均回收率分别为100.75%,RSD=1.19%和98.35%,RSD=1.46%（n=9）。结论：该方法精密度高,分离度好,可为该药的全面质量评价提供参考。Objective： To establish a simultaneous determination method for gallic acid and piperine in Naru Sanwei pills. Methods： The separation was carried out on an Ultimate XB-C18 （250 mm ×4.6 mm,5 μm） column. The mobile phase was methanol and 0.1% phosphoric acid with gradient elution. The gradient program was as follows： in 0-20 min, methanol was changed from 15% to 80% ; in 20-30 min, methanol was changed from 80% to 15%. The flow rate was 1.0 ml · min-1. The detection wavelength was 285 nm, the column temperature was 30℃ ,and the sample size was 10 p,1. Results： The linear range of gallic acid and piperine was 0.878- 4. 392 μg（r = 1. 000 0）and 0. 262-1. 308 μg（r = 1. 000 0）, respectively. The average recovery was 100.75% （RSD = 1.19% ） and 98.35% （RSD = 1.46% ） （ n = 9）, respectively. Conclusion： The method with high precision and resolution can be used for the quality control of Naru Sanwei pills.

关 键 词：高效液相色谱法 那如三味丸 没食子酸 胡椒碱 

分 类 号：R927.2[医药卫生—药学]