卵巢上皮性癌患者外周血中septin-9和clusterin蛋白的表达及其意义  

Protein levels and its clinical significance of septin-9 and clusterin in peripheral blood of epithelial ovarian cancer patients

作　　者：吕讷男[1] 姚洪文[1] 肖汀[1] 高燕宁[1] 吴令英[1] 

机构地区：[1]中国医学科学院北京协和医学院肿瘤医院妇瘤科,100021

出　　处：《中华妇产科杂志》2015年第9期679-684,共6页Chinese Journal of Obstetrics and Gynecology

基　　金：基金项目：首都医科大学基础一临床科研合作基金（13JL20、13JL69）;首都医科大学附属北京妇产医院科研基金（201208）

摘　　要：目的：检测卵巢上皮性癌（卵巢癌）患者外周血中septin-9、clusterin蛋白的表达，并探讨其临床意义。方法收集2008年1月29日至2010年2月1日间中国医学科学院北京协和医学院肿瘤医院收治的200例患者的临床病理资料，其中137例卵巢癌（卵巢癌组）、12例卵巢交界性上皮性肿瘤（交界性肿瘤组）、10例卵巢良性上皮性肿瘤（良性肿瘤组）、41例盆腔良性病变（盆腔良性病变组），以同期进行健康体检的58例健康妇女作为对照组，采用双抗体夹心法ELISA和ELISA法分别检测各组患者血浆中septin-9、clusterin蛋白的表达；分析血浆中septin-9、clusterin蛋白表达的临床意义；并以受试者工作特征（ROC）曲线的曲线下面积（AUC）评估血浆中septin-9、clusterin蛋白检测对卵巢癌的诊断效能。结果双抗体夹心法ELISA法检测显示，卵巢癌组和盆腔良性病变组患者外周血中septin-9蛋白表达水平（A值分别为0.35±0.09、0.36±0.09）明显高于对照组（A值为0.31±0.12，P〈0.01）；而交界性肿瘤组、良性肿瘤组（A值分别为0.30±0.04、0.33±0.04）分别与对照组比较，差异则无统计学意义（P〉0.05）。卵巢癌患者外周血中septin-9蛋白表达水平与有无肿瘤家族史（A值分别为0.38±0.10、0.34±0.08）和远处转移（A值分别为0.37±0.10、0.34±0.08）有关（P〈0.05），而与年龄、手术病理分期、病理分化程度以及有无吸烟史、治疗史（包括手术、放化疗）和淋巴结转移均无关（P〉0.05）。ELISA法检测显示，卵巢癌组患者外周血中clusterin蛋白表达水平明显高于对照组[分别为（109±52）、（103±18）pg/L，P=0.021]；而交界性肿瘤组、良性肿瘤组、盆腔良性病变组[分别为（88±41）、（91±23）、（94±29）pg/L]分别与对照组比较，差异则均无统计学意义（P〉0.05）。卵巢癌患者外周血中clusterin蛋白表达水平与上述临床病理指Objective To evaluate septin-9 and clusterin protein levels in the peripheral blood samples from epithelial ovarian cancer patients, and explore its clinical significance. Methods Clinical data of 200 patients in Cancer Hospital,Chinese Academy of Medical Sciences from Jan. 29, 2008 to Feb. 1,2010 were collected. The peripheral blood samples were obtained from 137 epithelial ovarian cancer patients, 12 borderline ovarian tumor patients, 10 benign ovarian tumor patients, 41 benign pelvic lesion patients and 58 healthy women. The septin-9 and clusterin protein levels in the plasma were measured by double antibody sandwich ELISA or ELISA. The clinical significance of clusterin and septin-9 in plasma was analyzed. The diagnostic efficacy of septin-9 and clusterin protein in the detection of ovarian cancer was evaluated by the area under the curve （AUC） of the receiver operating characteristic （ROC） curve. Results Double antibody sandwich ELISA showed： the mean levels of plasma septin-9 in epithelial ovarian cancer patients or benign pelvic lesion patients were significantly higher than that in healthy women detedted by double antibody sandwich ELISA （P〈0.01）. The mean levels of plasma septin-9 in epithelial ovarian carcinoma patients with tumor family history or distance metastasis were significantly higher than those patients without （P〈0.05）. While the expression level of septin-9 protein in peripheral blood of ovarian cancer patients was not related to the patient age, pathologic stage, pathologic differentiation, smoking history, treatment history （including surgery, radiotherapy and chemotherapy） and lymph node metastasis （all P〉0.05）. ELISA showed： the mean level of plasma clusterin in epithelial ovarian cancer patients was significantly higher than that in healthy women deteded by ELISA （P=0.021）. The expression level of clusterin protein in peripheral blood of ovarian cancer patients was not related to the above clinical pathological parameters （all P〉0.05）. To dist

关 键 词：卵巢肿瘤 氯苄乙胺 聚集素 酶联免疫吸附测定 

分 类 号：R737.31[医药卫生—肿瘤]